Interferon production by the preimplantation sheep embryo.
Interferon production by the preimplantation sheep embryo.
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植入前绵羊胚胎产生干扰素。
DOI：
10.1089/jir.1989.9.175
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Kronenberg,LH
中科院分区：
文献类型：
--
作者：
Roberts,RM;Imakawa,K;Niwano,Y;Kazemi,M;Malathy,PV;Hansen,TR;Glass,AA;Kronenberg,LH
绵羊滋养层蛋白-1（oTP-1）是绵羊妊娠第13 ~ 21天胚胎滋养外胚层分泌的主要产物，通过维持黄体功能介导母体对妊娠的识别。其氨基酸序列与多种哺乳动物干扰素-α（IFN-α）具有40-55%的同源性，并且已显示其具有抗病毒活性。目前的结果证实了oTP-1是一种功能性IFN，其在妊娠15-17天由单个胚胎产生，每天超过100 μg（> 100万抗病毒单位）。它的氨基末端序列表明它由单一的同质蛋白质组成，因此它的抗病毒活性可能不是由于污染物。用水泡性口炎感染的GBK-2牛细胞进行细胞病变抑制试验，其比活性为1.3 × 107个终点单位/mg蛋白。它还保护GBK-2细胞免受其他四种病毒的侵害，并保护A549人类细胞免受脑心肌炎病毒的侵害。抗病毒活性被人白细胞IFN-α的抗血清中和。与人IFN-α一样，oTP-1在低至10− 9 M的浓度下抑制培养的GBK细胞的生长，并抑制有丝分裂原刺激的[3 H]胸苷掺入绵羊淋巴细胞。
Ovine trophoblast protein-1 (oTP-1), the major product secreted by the trophectoderm of the sheep conceptus between days 13 and 21 of pregnancy, is considered to mediate maternal recognition of pregnancy by maintaining the function of the corpus luteum. Its amino acid sequence has 40–55% identity with various mammalian interferons-α (IFN-α), and it has been shown to have antiviral activity. The present results confirm that oTP-1, which at days 15–17 of pregnancy is produced by a single embryo at more than 100 μg (>1 million antiviral units) per day, is a functional IFN.A preparation of purified oTP-1 was made. Its amino-terminal sequence suggested that it consisted of a single homogeneous protein, so that its antiviral activity probably was not due to a contaminant. In a cytopathic effect inhibition assay with GBK-2 bovine cells challenged with vesicular stomatitis, its specific activity was 1.3 × 107end point units/mg protein. It also protected GBK-2 cells against four other viruses, and A549 human cells against encephalomyocarditis virus. The antiviral activity was neutralized by an antiserum to human leukocyte IFN.Like human IFN-α, oTP-1 at concentrations as low as 10−9M inhibited the growth of GBK cells in culture and suppressed mitogen-stimulated incorporation of [3H]thymidine into ovine lymphocytes.Possible roles for oTP-1, functioning as an IFN-α during early pregnancy, are discussed.
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影响因子：
3.4
作者：
C. Plante;P. Hansen;M. Mirando;W. Thatcher;F. Bazer
通讯作者：
F. Bazer
干扰素-α 在体外模拟绵羊滋养层蛋白 1 对绵羊子宫内膜细胞前列腺素和蛋白质分泌的影响。
DOI：
10.1677/joe.0.117r001
发表时间：
1988
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Salamonsen,LA;Stuchbery,SJ;O'Grady,CM;Godkin,JD;Findlay,JK
通讯作者：
Findlay,JK
牛子宫分泌物抑制白细胞介素 2 介导的 T 淋巴细胞母细胞发生
影响因子：
3.6
作者：
E. C. Segerson;F. C. Gunsett
通讯作者：
F. C. Gunsett
山羊滋养层蛋白-1 的 N-糖基化和非糖基化形式由植入前山羊受孕者分泌。
DOI：
--
发表时间：
1990
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
G. Baumbach;R. Duby;J. Godkin
通讯作者：
J. Godkin
影响因子：
4.8
作者：
B. Velan;S. Cohen;H. Grosfeld;M. Leitner;A. Shafferman
通讯作者：
A. Shafferman