Phage-antibiotic synergism: a possible approach to combatting Pseudomonas aeruginosa
Phage-antibiotic synergism: a possible approach to combatting Pseudomonas aeruginosa
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DOI：
10.1016/j.resmic.2012.08.008
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Vlaski, Ljiljana
中科院分区：
文献类型：
--
作者：
Knezevic, Petar;Curcin, Sanja;Vlaski, Ljiljana
铜绿假单胞菌是一种高度耐药的条件致病菌，是各种感染的重要病原。在过去的十年中，铜绿假单胞菌噬菌体已被广泛研究作为替代抗菌剂。本研究的目的是确定亚抑制浓度的庆大霉素、头孢曲松、环丙沙星或多粘菌素B与绿脓杆菌属Podoviridae和Siphoviridae的铜绿假单胞菌特异性噬菌体联合使用的抗菌效果。时间杀伤曲线法显示，噬菌体与头孢曲松亚抑制浓度的联合作用普遍降低了细菌的生长，并在孵育300 min后证明了对Siphoviridae噬菌体sigma-1的协同作用。应用头孢曲松后检测到的形态学改变，导致细胞伸长，以及其特定的作用方式，似乎是噬菌体-抗生素协同作用的必要但不是充分的原因。这一现象为今后发展由铜绿假单胞菌引起的潜在致命感染的治疗策略提供了机会。(C) 2012巴斯德研究所。Elsevier Masson SAS出版。版权所有。
Pseudomonas aeruginosa is a highly resistant opportunistic pathogen and an important etiological agent of various types of infections. During the last decade, P. aeruginosa phages have been extensively examined as alternative antimicrobial agents. The aim of the study was to determine antimicrobial effectiveness of combining subinhibitory concentrations of gentamicin, ceftriaxone, ciprofloxacin or polymyxin B with P. aeruginosa-specific bacteriophages belonging to families Podoviridae and Siphoviridae. The time-kill curve method showed that a combination of bacteriophages and subinhibitory concentrations of ceftriaxone generally reduced bacterial growth, and synergism was proven for a Siphoviridae phage sigma-1 after 300 min of incubation. The detected alteration in morphology after ceftriaxone application, resulting in cell elongation, along with its specific mode of action, seemed to be a necessary but was not a sufficient reason for phage-antibiotic synergism. The phenomenon offers an opportunity for future development of treatment strategies for potentially lethal infections caused by P. aeruginosa. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.