Understanding Dichromic Fluorescence Manifested in Certain ICG Analogs.
Understanding Dichromic Fluorescence Manifested in Certain ICG Analogs.
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DOI：
10.1351/pac-con-08-12-07
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Achilefu S
中科院分区：
文献类型：
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作者：
Zhang Z;Kao J;D'Avignon A;Achilefu S
荧光促进了我们对生物过程各个方面的理解。近红外（NIR）区域中的荧光避免了来自生物样品的背景自发荧光，从而提高了图像质量。在寻找可与生物分子连接的吲哚菁绿色（ICG）类似物的过程中，我们观察到一些单反应基团功能化的ICG类似物表现出二色荧光。这两个发射带彼此明显分开，使其成为生物研究中发现的荧光探针的独特特征。我们进一步证明了二色性来自结构，并且可以从染料转移到其生物缀合物。在本文中，我们使用共振理论和分子轨道理论来解释荧光团的光化学，努力了解一般的荧光特性的ICG类似物，并提供了理解的次级发射带。
Fluorescence has advanced our understanding in various aspects of biological processes. Fluorescence in the near infrared (NIR) region avoids background autofluorescence from biological samples leading to improved image quality. In searching for indocyanine green (ICG) analogs that can be attached to biomolecules, we observed that dichromic fluorescence manifested in some mono reactive-group functionalized ICG analogs. The two emission bands are distinctively separate from each other, making it a unique feature of fluorescent probes found in biological studies. We further demonstrated that the dichromism comes from the structure and is transferable from dye to its bioconjugates. In this paper, we used Resonance Theory and Molecular Orbital Theory to explain the fluorophore photochemistry in an effort to understand the general fluorescence feature of ICG analogs and provide understanding of the secondary emission band.