Corrigendum: Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB.
Corrigendum: Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB.
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DOI：
10.1038/ncomms13768
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发表时间：
2016-11-23
影响因子：
16.6
通讯作者：
Meunier FA
中科院分区：
文献类型：
--
作者：
Wang T;Martin S;Nguyen TH;Harper CB;Gormal RS;Martínez-Mármol R;Karunanithi S;Coulson EJ;Glass NR;Cooper-White JJ;van Swinderen B;Meunier FA
信号内体从神经末梢到体细胞的轴突逆行运输支撑着生存。由于每个信号内体都携带一定数量的激活受体，我们假设内体到达胞体的频率决定了营养信号的规模。在这里，我们发现，上调突触活性显着增加了微流体装置中培养的海马神经元和活果蝇幼虫运动神经元中质膜衍生的逆行内体（使用霍乱毒素亚基-B：CTB标记）的通量。电子和超分辨率显微镜分析显示，快速移动的亚衍射限制的 CTB 载体含有 TrkB 神经营养蛋白受体，该受体以不依赖 BDNF 的方式被突触活动瞬时激活。 TrkB 激活的药理学和遗传抑制选择性地阻止突触活性和信号内体逆行流之间的耦合。因此，TrkB 活性控制着神经末梢所经历的突触活动的编码，并数字化为逆行运输的信号内体的通量。
Axonal retrograde transport of signalling endosomes from the nerve terminal to the soma underpins survival. As each signalling endosome carries a quantal amount of activated receptors, we hypothesized that it is the frequency of endosomes reaching the soma that determines the scale of the trophic signal. Here we show that upregulating synaptic activity markedly increased the flux of plasma membrane-derived retrograde endosomes (labelled using cholera toxin subunit-B: CTB) in hippocampal neurons cultured in microfluidic devices, and liveDrosophilalarval motor neurons. Electron and super-resolution microscopy analyses revealed that the fast-moving sub-diffraction-limited CTB carriers contained the TrkB neurotrophin receptor, transiently activated by synaptic activity in a BDNF-independent manner. Pharmacological and genetic inhibition of TrkB activation selectively prevented the coupling between synaptic activity and the retrograde flux of signalling endosomes. TrkB activity therefore controls the encoding of synaptic activity experienced by nerve terminals, digitalized as the flux of retrogradely transported signalling endosomes.