Induction of cell-membrane porosity by ultrasound
Induction of cell-membrane porosity by ultrasound
复制标题
DOI：
10.1016/s0140-6736(99)01244-1
复制
发表时间：
1999-04-24
期刊：
影响因子：
168.9
通讯作者：
Tamura, K
中科院分区：
文献类型：
--
作者：
Tachibana, K;Uchida, T;Tamura, K
细胞内有声能。这些治疗的细胞杀伤机制尚未明确确定。为了通过超声识别肿瘤细胞损伤的位置和程度，我们用扫描电子显微镜研究了细胞表面的结构。将超声发射换能器（6× 6× 0.62 mm）直接插入HL-60细胞系悬液（3.0 × 106cells /mL）中。用连续波超声（255 kHz）在光敏药物merocyanine 540 （mc540: 15 μg/mL）存在下，以0.4 W/cm的强度对细胞进行超声处理
within cells with acoustic energy. The cell-killing mechanism of these treatments has not been clearly determined. To identify the site and degree of damage to tumour cells by sonication, we studied the structure of the cell surface with scanning electron microscopy.An ultrasound emitting transducer (6× 6× 0· 62 mm) was inserted directly into the HL-60 cell line suspension (3· 0× 10 6 cells/mL). Cells were sonicated with continuous wave ultrasound (255 kHz) in the presence of the photosensitive drug merocyanine 540 (MC 540: 15 μg/mL) at an intensity of 0· 4 W/cm