Detection of Rhizopus-specific antigen in human and murine serum and bronchoalveolar lavege.
Detection of Rhizopus-specific antigen in human and murine serum and bronchoalveolar lavege.
复制标题
人和鼠血清和支气管肺泡灌洗液中根霉特异性抗原的检测。
DOI：
10.1093/mmy/myaa001
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发表时间：
2020
期刊：
影响因子：
2.9
通讯作者：
Kakeya H
中科院分区：
文献类型：
--
作者：
Shibata W;Niki M;Sato K;Fujimoto H;Yamada K;Watanabe T;Miyazaki Y;Asai K;Obata Y;Taro T;Kawaguchi T;Kaneko Y;Kakeya H
毛霉菌病是一种深部真菌感染，主要发生于患有严重免疫缺陷的患者，例如患有恶性血液病的患者。尽管预后不佳，但尚无可靠的微创诊断方法（例如血清诊断）来做出有关该疾病的临床决策。由于早期诊断和早期治疗可改善毛霉菌病的预后，因此开发敏感的早期诊断方法非常重要。我们之前通过信号序列捕获和逆转录病毒介导的表达（SST-REX）鉴定了根霉特异性抗原（RSA），并通过构建夹心酶联免疫吸附测定（ELISA）系统来检测接种小鼠的血清RSA水平，评估了其作为诊断抗原的效用。在本研究中，我们使用新型杂交瘤技术产生的RSA特异性兔单克隆抗体来提高ELISA系统的灵敏度。我们观察到接种后 1 天小鼠模型的血清和支气管肺泡灌洗液 (BALF) RSA 水平增加，表明这种新开发的基于单克隆抗体的 ELISA 系统可能有助于毛霉菌病感染早期的诊断。此外，我们测量了人血清和 BALF 中的 RSA 水平，发现毛霉菌病患者的血清 RSA 水平（15.1 ng/ml）高于侵袭性肺曲霉病患者（0.53 ng/ml）和阴性对照（0.49 ng/ml）。我们的结果表明，RSA 可能是诊断肺毛霉菌病及其与曲霉病等其他深部真菌病的有力工具。
Mucormycosis is a deep-seated fungal infection that mainly develops in patients with severe immunodeficiencies such as those with malignant hematological diseases. Despite poor prognosis, there is no reliable and minimally invasive diagnostic method—such as serodiagnosis—for making a clinical decision regarding the condition. As early diagnosis and early treatment improve the prognosis of mucormycosis, the development of a sensitive early diagnostic method is important. We had previously identified aRhizopus-specific antigen (RSA) by signal sequence trapping and retrovirus-mediated expression (SST-REX), and evaluated its utility as a diagnostic antigen by constructing a sandwich enzyme-linked immunosorbent assay (ELISA) system to detect serum RSA levels in inoculated mice. In this study, we used the RSA-specific rabbit monoclonal antibodies generated by novel hybridoma technology to improve the sensitivity of the ELISA system. We observed an increase in serum and bronchoalveolar lavage fluid (BALF) levels of RSA in mouse model 1 day after inoculation, suggesting that this newly developed monoclonal antibody-based ELISA system may be useful for the diagnosis of mucormycosis in the early stages of infection. In addition, we measured RSA levels in human serum and BALF, and found that serum RSA level was higher in mucormycosis patients (15.1 ng/ml) than that in invasive pulmonary aspergillosis patients (0.53 ng/ml) and the negative control (0.49 ng/ml). Our results suggest that RSA may be a powerful tool for the diagnosis of pulmonary mucormycosis, and its differentiation from other deep-seated mycoses such as aspergillosis.