Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia
Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia
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DOI：
10.1099/0022-1317-80-1-179
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发表时间：
1999-01-01
影响因子：
3.8
通讯作者：
Heinz, FX
中科院分区：
文献类型：
--
作者：
Ecker, M;Allison, SL;Heinz, FX
通过对欧洲和亚洲流行地区分离的病毒株进行比较序列分析，研究了蜱传脑炎病毒的流行病学。根据 16 个新测序菌株和 9 个先前发表的序列的主被膜 (E) 蛋白的核苷酸和氨基酸序列确定了系统发育关系。可以清楚地区分三种遗传谱系，分别对应欧洲亚型、远东亚型和西伯利亚亚型。鉴定了每种亚型的特征氨基酸（“特征”氨基酸），并确定了它们在蛋白质 E 原子结构中的位置。亚型内菌株之间的变异程度较低，在氨基酸水平上最多仅表现出2.2%。三种亚型之间的最大差异为 5.6%，这在其他黄病毒报告的变异范围内。
The epidemiology of tick-borne encephalitis virus was investigated by comparative sequence analysis of virus strains isolated in endemic areas of Europe and Asia. Phylogenetic relationships were determined from the nucleotide and amino acid sequences of the major envelope (E) protein of 16 newly sequenced strains and nine previously published sequences. Three genetic lineages could be clearly distinguished, corresponding to a European, a Far Eastern and a Siberian subtype. Amino acids characteristic for each of the subtypes ('signature' amino acids) were identified and their location in the atomic structure of protein E was determined. The degree of variation between strains within subtypes was low and exhibited a maximum of only 2.2% at the amino acid level. A maximum difference of 5.6% was found between the three subtypes, which is in the range of variation reported for other flaviviruses.