Testis cancer gene D40 expression and its relationship with clinicopathological features in infertile men
Testis cancer gene D40 expression and its relationship with clinicopathological features in infertile men
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不育男性睾丸癌基因D40表达及其与临床病理特征的关系
DOI：
10.1111/j.1442-2042.2010.02692.x
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发表时间：
2011
影响因子：
2.6
通讯作者：
Takumi Sasao Masato Takimoto Naoki Itoh Toshihiro Maeda Toshiaki Tanaka Naoya Masumori and Taiji Tsukamoto
中科院分区：
文献类型：
--
作者：
Isao Kosugi;Yoshifumi Arai;Hideya Kawasaki;Yoshihiro Tsutsui;Takumi Sasao Masato Takimoto Naoki Itoh Toshihiro Maeda Toshiaki Tanaka Naoya Masumori and Taiji Tsukamoto
我们以前发现了一个新的人类癌症/睾丸基因，D40，它主要在睾丸生殖细胞，各种癌细胞系和原发性人类肿瘤中表达。采用常规逆转录聚合酶链反应（RT-PCR）、真实的实时定量RT-PCR和western blot分析定量各种睾丸组织中D40 mRNA和蛋白的表达。检测D40表达水平、血清促卵泡激素（FSH）水平与Johnsen评分的关系。在不育男性的睾丸中观察到D40 mRNA表达，只有支持细胞综合征或Klinefelter综合征的患者除外。D40 mRNA和蛋白表达量与Johnsen评分呈正相关，与血清FSH水平呈负相关。本研究结果表明，在男性不育患者中，D40 mRNA和蛋白的表达水平随着精子发生障碍的程度而降低。
We previously identified a novel human cancer/testis gene, D40, which is dominantly expressed in testicular germ cells, various cancer cell lines and primary human tumors. The expression of D40 mRNA and proteins in various testicular tissues was quantified using the conventional reverse transcription polymerase chain reaction (RT–PCR), real‐time quantitative RT–PCR and western blot analysis. The relationship between levels of D40 expression, serum follicle stimulating hormone (FSH) level and Johnsen's score was examined. D40 mRNA expression was observed in the testes of infertile men, except those with Sertoli‐cell‐only syndrome or Klinefelter's syndrome. The quantity of D40 mRNA and protein was correlated with Johnsen's score and inversely correlated with serum FSH level. The present results show that the expression levels of D40 mRNA and proteins decrease according to the degree of spermatogenesis impairment in male infertile patients.