Cloning of the feline GADD45 cDNA and analysis of its mutation in feline lymphoma cell lines.
Cloning of the feline GADD45 cDNA and analysis of its mutation in feline lymphoma cell lines.
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DOI：
10.1292/jvms.68.297
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发表时间：
2006-03
期刊：
影响因子：
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通讯作者：
T. Oikawa;M. Okuda;N. Kaneko;Malaika Watanabe;H. Hiraoka;K. Itamoto;M. Nakaichi;T. Mizuno;H. Inokuma
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文献类型：
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作者：
T. Oikawa;M. Okuda;N. Kaneko;Malaika Watanabe;H. Hiraoka;K. Itamoto;M. Nakaichi;T. Mizuno;H. Inokuma
生长停滞和 DNA 损伤诱导蛋白 45 (GADD45) 在抑制多步致癌过程中发挥着重要作用。在本报告中，我们描述了从猫组织中分离出完整的野生型猫 GADD45 cDNA。在猫的肝、脾、肾、肺和睾丸中检测到 GADD45 mRNA 的表达。由全长猫科动物GADD45 cDNA编码的预测氨基酸序列显示出与其他脊椎动物的序列同源性，并且与人GADD45的情况一样，通过猫科动物GADD45的异位表达观察到细胞生长抑制。然而，通过对几种猫淋巴瘤细胞系中猫GADD45的序列分析未检测到突变，表明GADD45突变在猫肿瘤发生中可能不常见。
Growth arrest and DNA damage-inducible protein 45 (GADD45) plays an important role in suppressing multistep carcinogenesis. In this report, we describe the isolation of the complete wild-type feline GADD45 cDNA from feline tissues. Expression of feline GADD45 mRNA was detected in the liver, spleen, kidney, lung, and testis. The predicted amino acid sequences encoded by the full-length feline GADD45 cDNA display sequence homology with those from other vertebrates, and as in the case of human GADD45, cell growth suppression was observed by ectopic expression of feline GADD45. However, no mutations were detected by sequence analysis of feline GADD45 in several feline lymphoma cell lines, indicating that the GADD45 mutation might be uncommon in feline oncogenesis.