Genomic organization of the 70-kDa peroxisomal membrane protein gene (PXMP1).
Genomic organization of the 70-kDa peroxisomal membrane protein gene (PXMP1).
复制标题
70-kDa 过氧化物酶体膜蛋白基因 (PXMP1) 的基因组结构。
DOI：
10.1006/geno.1997.5177
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Valle,D
中科院分区：
文献类型：
--
作者：
Gartner,J;Jimenez-Sanchez,G;Roerig,P;Valle,D
70-kDa过氧化物酶体膜蛋白（PMP 70）是位于人过氧化物酶体膜上的半ATP结合盒（ABC）转运蛋白家族的成员。其他成员包括PMP 70相关的过氧化物酶体膜蛋白、肾上腺脑白质营养不良蛋白（ALDP）和肾上腺脑白质营养不良相关蛋白。ABC转运蛋白在过氧化物酶体膜中的功能知之甚少。来自酵母和人类突变体的证据表明，它们参与脂肪酸和/或脂肪酰辅酶A进入细胞器的过氧化物酶体进口。本文报道了人PMP 70结构基因（基因符号：PXMP 1）的克隆和鉴定，该基因位于人染色体1 p21-p22，长约65 kb，含有23个外显子，与编码ALDP的基因（ALD）结构完全不同。对人PXMP-1基因5′侧翼区和鼠Pxmp-1基因5′侧翼区的序列进行了分析，发现两个启动子都具有管家基因的特征，包括GC含量高和多个Sp1结合序列。在超过3 kb的Pxmp-15′侧翼序列中，我们没有鉴定出共有的过氧化物酶体增殖物反应元件。
The 70-kDa peroxisomal membrane protein (PMP70) is a member of a family of half-ATP-binding cassette (ABC) transporter proteins located in the human peroxisomal membrane. Other members include the PMP70-related peroxisomal membrane protein, the adrenoleukodystrophy protein (ALDP), and the adrenoleukodystrophy-related protein. The functions of ABC transporters in the peroxisomal membrane are poorly understood. Evidence from yeast and human mutants suggests that they are involved in the peroxisomal import of fatty acids and/or fatty acyl-CoAs into the organelle. We report the cloning and characterization of the human PMP70 structural gene (gene symbol:PXMP1) localized on human chromosome 1p21–p22.PXMP1is approximately 65 kb in length, contains 23 exons, and is quite different in structure from the gene (ALD) that encodes the related protein, ALDP. We also analyzed the 5′ flanking region of the humanPXMP1gene and the corresponding region of murinePxmp-1.Both promoters have features of housekeeping genes, including a high GC content and multiple consensus Sp1 binding sequences. In more than 3 kb ofPxmp-15′ flanking sequence we did not identify a consensus peroxisomal proliferator responsive element.