SINGLE CELL RECORDINGS FROM TSETSE (GLOSSINA M-MORSITANS) ANTENNAE REVEAL OLFACTORY, MECHANO AND COLD RECEPTORS
SINGLE CELL RECORDINGS FROM TSETSE (GLOSSINA M-MORSITANS) ANTENNAE REVEAL OLFACTORY, MECHANO AND COLD RECEPTORS
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DOI：
10.1111/j.1365-3032.1992.tb00987.x
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发表时间：
1992-03-01
影响因子：
1.5
通讯作者：
VANNATERS, WMV
中科院分区：
文献类型：
--
作者：
DENOTTER, CJ;VANNATERS, WMV
从单个细胞的动作电位和受体电位记录从触角（索）的活采采蝇，Glossina m.morsitans Westwood，使用“表面接触”记录技术。刺激是电极的压力，温度的变化和1-辛烯-3-醇，丙酮，3-甲基苯酚，二氯甲烷和CO2的蒸气。结果发现，在压力刺激下，两种类型的机械感受性细胞只产生动作电位，而在压力刺激下，两种类型的机械感受性细胞则持续产生动作电位。在测试其对温度变化和气味刺激的敏感性的182个细胞中，19%对任何刺激都没有反应，这表明我们仍然不知道可能对苍蝇有重要意义的线索，4%只对温度有反应，分别随着温度的降低和增加而增加和减少它们的活性。在141个嗅觉受体细胞中，52%对1-辛烯-3-醇有反应，40%对该物质完全敏感。对于3-甲基苯酚，这些百分比为23和18，对于丙酮为13和11，对于二氯甲烷为12和7，对于CO2为13和13。在索的某些区域没有发现细胞类型的聚集，两性之间也没有明显的差异。大多数嗅觉细胞的反应是增加它们对气味刺激的活动。仅在三个细胞中发现抑制，其中自发活动在用3-甲基苯酚刺激时受到抑制。棘波反应是相位紧张性的，在两个极端之间变化：刺激结束后，细胞显示出相对快速的棘波活动停止，刺激后持续放电数秒或数分钟的细胞。各种细胞类型的活动可能的行为影响进行了讨论。
Action potentials from individual cells and receptor potentials were recorded from antennae (funiculi) of living tsetse flies, Glossina m.morsitans Westwood, using a 'surface-contact' recording technique. Stimuli were pressure of the electrode, changes in temperature and the vapours of 1-octen-3-ol, acetone, 3-methylphenol, dichloromethane and CO2. Two types of mechanoreceptive cells were found. One type only fired action potentials when pressure was increased, the other type continued firing when pressure was maintained. Of the 182 cells tested for their sensitivity to temperature change and the odour stimuli, 19% did not respond to any of the stimuli, suggesting that we are still unaware of cues which may be of import to the flies, and 4% responded to temperature only, increasing and decreasing their activity with a decrement and increment in temperature, respectively. Of the 141 cells which were olfactory receptors, 52% responded to 1-octen-3-ol and 40% were exclusively sensitive to this substance. For 3-methylphenol these percentages were 23 and 18, for acetone 13 and 11, for dichloromethane 12 and 7, for CO2 13 and 13. No clustering of cell types in certain areas of the funiculi was found, nor was a difference apparent between the sexes. The majority of the olfactory cells responded by increasing their activities on odour stimulation. Inhibition was found in three cells only, in which spontaneous activity was suppressed on stimulation with 3-methylphenol. Spike responses were phasic-tonic and varied between two extremes: Cells showing relatively rapid cessation of spike activity after the end of stimulation, and cells which continued firing for several seconds or minutes after stimulation. Possible behavioural effects of the activities of the various cell types are discussed.