Effects of 2.45 GHz microwave fields on liposomes entrapping glycoenzyme ascorbate oxidase: Evidence for oligosaccharide side chain involvement
Effects of 2.45 GHz microwave fields on liposomes entrapping glycoenzyme ascorbate oxidase: Evidence for oligosaccharide side chain involvement
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2.45 GHz 微波场对包埋糖酶抗坏血酸氧化酶的脂质体的影响：寡糖侧链参与的证据
DOI：
10.1002/bem.10203
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发表时间：
2004
影响因子：
1.9
通讯作者：
G. D'Inzeo
中科院分区：
文献类型：
--
作者：
A. Ramundo‐Orlando;M. Liberti;G. Mossa;G. D'Inzeo
本研究组先前的观察结果表明，2.45 GHz的微波场在比吸收率（SAR）为5.6 W/kg的降低酶活性率的抗坏血酸氧化酶（AO）被困在脂质体。在本研究中，我们报告了在不同SAR值（1.4、2.8、4.2和5.6 W/kg）下辐照的这些含AO脂质体的剂量反应研究。SAR低于5.6 W/kg时未观察到任何反应。还使用了以其去糖化形式（AO-D）包埋功能性AO的脂质体。在这种情况下，没有观察到MW相关的酶活性变化，表明AO的寡糖链直接参与。此外，AO和AO-D的催化性能均未受到MW辐照的损害，无论是在均匀溶液中还是在脂质体中，排除了酶构象的可能变化作为一种机制。我们的研究结果表明，寡糖链的AO是至关重要的，引发微波观察到的影响脂质膜。生物电磁学25：338-345，2004年。© 2004 Wiley利斯公司
Previous observations reported by our group indicate that 2.45 GHz microwave fields at specific absorption rate (SAR) of 5.6 W/kg reduce the enzyme activity rate of ascorbate oxidase (AO) trapped in liposomes. In this study, we report dose‐response studies on these AO containing liposomes irradiated at different SAR values (1.4, 2.8, 4.2, and 5.6 W/kg). No response was observed for SAR below 5.6 W/kg. Liposomes entrapping functional AO in its deglycated form (AO‐D) were also used. In this case, no MW related enzyme activity changes were observed, demonstrating a direct involvement of oligosaccharide chains of AO. Furthermore, the catalytic properties of both AO and AO‐D were not impaired by MW irradiation, neither in homogeneous solution nor loaded in liposomes, excluding possible changes in the conformation of enzyme as a mechanism. Our results suggest that the oligosaccharide chains of AO are critical to elicit the microwave observed effects on lipid membrane. Bioelectromagnetics 25:338–345, 2004. © 2004 Wiley‐Liss, Inc.