AUDITORY EPITHELIAL MIGRATION .3. DEVELOPMENT OF THE STRATIFIED SQUAMOUS EPITHELIUM OF THE TYMPANIC MEMBRANE AND EXTERNAL CANAL IN THE MOUSE
AUDITORY EPITHELIAL MIGRATION .3. DEVELOPMENT OF THE STRATIFIED SQUAMOUS EPITHELIUM OF THE TYMPANIC MEMBRANE AND EXTERNAL CANAL IN THE MOUSE
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DOI：
10.1002/aja.1001910307
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发表时间：
1991-07-01
影响因子：
--
通讯作者：
SOUCEK, S
中科院分区：
文献类型：
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作者：
MICHAELS, L;SOUCEK, S
本文对124只胎龄11 ~ 100天的小鼠耳鼓膜和外耳道复层鳞状上皮的发育进行了连续切片观察。从初生根管底部边缘开始形成褶皱。它有两个区域：首先是耳道板，其产生了覆盖耳道部的上皮（区2）和大部分的深部耳道上皮（区3），其次是从不形成耳道板的底部部分生长的底部延伸板。基底延伸板产生了覆盖松弛部的上皮（1区），也产生了邻近的深管上皮（3区）。与人类发育不同的是，小鼠的3区，在口板和底延伸板衍生的区域，形成附属结构。在口板的早期发育中，区3，在其尖端，肿胀和积极的有丝分裂，并一直延伸到区2侧的一小段距离。第2区在第3区后两天首次被感知，逐渐变弱，到第4天形成一个单一的薄层。这表明，3区的近端部分，位于周围的鼓膜周围的成熟的耳朵，是一个产生中心的单向向外流动的上皮细胞终止于小鼠的第一附属器结构。通过负接触抑制，它可以使整个2区向同一方向运动。
The development of the stratified squamous epithelium of the tympanic membrane and external auditory canal was studied in serial sections of 124 mouse ears aged from 11 gestational days to 100 days. A fold developed from the edge of the fundus of the primary canal. It possessed two regions: firstly the meatal plate, which produced the pars tensa-covering epithelium (zone 2) and most of the deep ear canal epithelium (zone 3), and secondly the fundal extension plate, which grew from that part of the fundus not forming the meatal plate. The fundal extension plate gave rise to the pars flaccida-covering epithelium (zone 1) and also to the adjacent deep canal epithelium (zone 3). A difference from human development was that zone 3 in the mouse, in both the meatal plate- and the fundal extension plate-derived areas, formed adnexal structures. In the early development of the meatal plate, zone 3, at its tip, was swollen and actively mitotic and extended always for a short distance on to the zone 2 side. Zone 2, first perceived two days after zone 3, became progressively attenuated, and by the fourth day after its formation was a single thin layer. It is suggested that the proximal part of zone 3, situated in the mature ear around the periphery of the tympanic membrane, is a generation center for unidirectional outward flux of epithelium which terminates in the mouse at the first adnexal structure. It may cause the whole of zone 2 to move in the same direction by negative contact inhibition.