Differential response ofPhytophthora sojaezoospores to soybean seed exudates provides evidence of seed exudates participate in host resistance
Differential response ofPhytophthora sojaezoospores to soybean seed exudates provides evidence of seed exudates participate in host resistance
复制标题
大豆疫霉孢子对大豆种子分泌物的差异反应提供了种子分泌物参与寄主抗性的证据
DOI：
10.1007/s11104-020-04607-z
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发表时间：
2020
期刊：
影响因子：
4.9
通讯作者：
Wen Jingzhi
中科院分区：
文献类型：
--
作者：
Zhang Zhuoqun;Liu Haixu;Bi Xiangqi;Yu Han;Xu Ying;Chen Yufei;Yang Zhiyue;Wen Jingzhi
感病和抗病大豆品种Sloan和威廉姆斯82种子分泌物对土传病原大豆疫霉游动孢子的影响差异尚不清楚。本研究旨在阐明种子分泌物在大豆抗磷性中的作用。方法观察感染前的应激行为。大豆芽生孢子与种子分泌物的蛋白质组比较，结果表明，大豆芽生孢子与种子分泌物的蛋白质组差异显著。用TMT法分析了用大豆种子分泌物处理的大豆游动孢子。通过qRT-PCR定量分析关键蛋白基因的表达。大豆对这两种种子分泌物均表现出明显的趋化作用，其中Sloan种子分泌物对大豆的趋化作用较强。Sloan种子分泌物对游动孢子包囊形成的促进作用也强于威廉姆斯82。游动孢子中与趋化性相关的关键蛋白质在响应过程中表达上调，这些蛋白质响应Sloan的上调倍数高于威廉姆斯82。差异表达的蛋白质促进游动孢子的包囊形成。Sloan种子分泌物处理的雄鼠中这些蛋白质的表达变化是威廉姆斯82种子分泌物处理的雄鼠的几倍。大豆游动孢子暴露于两种大豆种子分泌物支持游动孢子响应两种种子分泌物的差异发育行为，如趋化性和包囊化。
AimsDifferential effects of the seed exudates of susceptible and resistant soybean cultivars Sloan and Williams 82 on the zoospores of soilborne pathogenicPhytophthora sojaeare not clear. This study aims to elucidate the role of the seed exudates in the host resistance of soybean againstP. sojaeduring prior to infection.MethodsThe response behaviors ofP. sojaezoospores to the seed exudates were performed in an assay chamber and concave slide, the proteomes ofP. sojaezoospores treated with the seed exudates were analyzed by TMT method. The expression of genes encoding key proteins was quantified by qRT-PCR.ResultsThe zoospores ofP. sojaeexhibited significant chemotaxis to the both seed exudates and a stronger attracted was found to the seed exudates of Sloan. The seed exudates of Sloan also had a stonger promotion than that of Williams 82 on encystment of zoospores. During the responding process, the key proteins in zoospores related to chemotaxis were upregulated, the upregulation fold of these proteins in responding to Sloan was higher than that of Williams 82. Proteins differentially expressed promote the encystment of zoospores. The changes in expression of these proteins was several fold higher in the zoosprores exposed to the seed exudates of Sloan than that of Williams 82.ConclusionsEvidence of differential changes in protein expression inP. sojaezoospores exposed to the seed exudates of both soybean supports the differential developmental behaviors such as chemotaxis and encystment of the zoospores in responding to the two seed exudates.