Differential binding to human mammary and nonmammary tumors of monoclonal antibodies reactive with carcinoembryonic antigen.
Differential binding to human mammary and nonmammary tumors of monoclonal antibodies reactive with carcinoembryonic antigen.
复制标题
与癌胚抗原反应的单克隆抗体与人乳腺和非乳腺肿瘤的差异结合。
DOI：
10.3109/07357908309042415
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发表时间：
1983
影响因子：
2.4
通讯作者：
J. Schlom
中科院分区：
文献类型：
--
作者：
D. Colcher;P. Hand;M. Nuti;J. Schlom
用人乳腺癌膜富集部分免疫的小鼠的脾淋巴细胞与 NS-1 非分泌性++骨髓瘤细胞系融合。筛选所得杂交瘤中与人乳腺肿瘤相关抗原反应的免疫球蛋白的合成，并鉴定出两种 IgG 单克隆抗体（B1.1 和 F5.5）与纯化的癌胚抗原（CEA）反应。这些抗体与 CEA 上的不同表位结合，基于它们对五种不同的纯化 CEA 制剂的不同反应性，以及它们与来自不同器官部位的肿瘤细胞表面的不同结合。单克隆 B1.1 与人乳腺、结肠和黑色素瘤细胞系的表面均等结合。另一方面，单克隆 F5.5 不与黑色素瘤细胞系表面发生反应，并且与乳腺癌细胞和结肠癌细胞的结合表现出差异。单克隆抗体 F5.5 和 B1.1 均用于免疫过氧化物酶研究，对多种组织学类型的人乳腺癌的固定组织切片进行研究，结果显示分别与 55% 和 66% 的肿瘤块发生反应。
Splenic lymphocytes of mice immunized with membrane enriched fractions of human mammary carcinomas were fused with the NS-1 nonsecretory++ myeloma cell line. The resulting hybridomas were screened for the synthesis of immunoglobulins reactive with human mammary tumor associated antigens, and two IgG monoclonal antibodies (B1.1 and F5.5) were identified as being reactive with purified carcinoembryonic antigen (CEA). These antibodies were shown to bind to different epitopes on CEA based on their differential reactivities to five different purified CEA preparations, and their differential binding to the surface of tumor cells derived from various organ sites. Monoclonal B1.1 bound equally to the surface of human breast, colon, and melanoma cell lines. Monoclonal F5.5, on the other hand, did not react with the surface of melanoma cell lines, and showed a differential binding to breast carcinoma versus colon carcinoma cells. Monoclonals F5.5 and B1.1 were both used in immunoperoxidase studies with fixed tissue sections of a variety of histologic types of human mammary carcinomas and were shown to be reactive with 55% and 66%, respectively, of tumor masses.