Adipocytes in both brown and white adipose tissue of adult mice are functionally connected via gap junctions: implications for Chagas disease
Adipocytes in both brown and white adipose tissue of adult mice are functionally connected via gap junctions: implications for Chagas disease
复制标题
DOI：
10.1016/j.micinf.2014.08.006
复制
发表时间：
2014-11-01
影响因子：
5.8
通讯作者：
Spray, David C.
中科院分区：
文献类型：
--
作者：
Burke, Shoshana;Nagajyothi, Fnu;Spray, David C.
脂肪组织是原生动物克氏锥虫的宿主，克氏锥虫是恰加斯病的病原体。间隙连接连接大多数组织的细胞，服务于同步细胞活动，包括分泌腺组织，我们以前已经证明，间隙连接改变在各种组织和细胞感染克氏锥虫。在此，我们研究了感染的脂肪组织中的差距连接蛋白连接蛋白43（Cx43）的表达。脂肪组织是人体最大的内分泌器官，也参与其他生理功能。在哺乳动物中，它主要由白色脂肪细胞组成。尽管缝隙连接是棕色脂肪细胞的显著特征，但在白色脂肪细胞中，尤其是在感染的情况下，尚未对其进行广泛研究。因此，我们研究了小鼠白色和棕色脂肪细胞的功能偶联。向脂肪组织内的脂肪细胞中注射电流或荧光黄染料会扩散到邻近的细胞，而用已知阻断间隙连接的药物治疗会减少这种扩散。此外，在棕色和白色脂肪组织中均检测到Cx43。在感染后30天和90天，Cx43在棕色脂肪细胞中下调，在白色脂肪细胞中上调。缝隙连接介导的细胞间通讯可能有助于激素分泌和其他功能的白色脂肪组织和非颤抖性产热的棕色脂肪，和调制的耦合克氏锥虫感染预计会影响这些功能。(C)2014由Elsevier Masson SAS代表巴斯德研究所出版。
Adipose tissue serves as a host reservoir for the protozoan Trypanosoma cruzi, the causative organism in Chagas disease. Gap junctions interconnect cells of most tissues, serving to synchronize cell activities including secretion in glandular tissue, and we have previously demonstrated that gap junctions are altered in various tissues and cells infected with T cruzi. Herein, we examined the gap junction protein connexin 43 (Cx43) expression in infected adipose tissues. Adipose tissue is the largest endocrine organ of the body and is also involved in other physiological functions. In mammals, it is primarily composed of white adipocytes. Although gap junctions are a prominent feature of brown adipocytes, they have not been explored extensively in white adipocytes, especially in the setting of infection. Thus, we examined functional coupling in both white and brown adipocytes in mice. Injection of electrical current or the dye Lucifer Yellow into adipocytes within fat tissue spread to adjacent cells, which was reduced by treatment with agents known to block gap junctions. Moreover, Cx43 was detected in both brown and white fat tissue. At thirty and ninety days post-infection, Cx43 was downregulated in brown adipocytes and upregulated in white adipocytes. Gap junction-mediated intercellular communication likely contributes to hormone secretion and other functions in white adipose tissue and to nonshivering thermogenesis in brown fat, and modulation of the coupling by T cruzi infection is expected to impact these functions. (C) 2014 Published by Elsevier Masson SAS on behalf of Institut Pasteur.