Aptamer:toxin conjugates that specifically target prostate tumor cells
Aptamer:toxin conjugates that specifically target prostate tumor cells
复制标题
DOI：
10.1158/0008-5472.can-05-4583
复制
发表时间：
2006-06-15
期刊：
影响因子：
11.2
通讯作者：
Levy, Matthew
中科院分区：
文献类型：
--
作者：
Chu, Ted C.;Marks, John W., III;Levy, Matthew
我们已经使用RNA适体：白树毒素缀合物靶向并特异性破坏过表达已知癌症生物标志物前列腺特异性膜抗原（PSMA）的细胞。适体：毒素缀合物具有27 nmol/L的IC 50，并且相对于不表达PSMA的细胞显示出至少600倍的增加的效力。适体不仅促进靶细胞的摄取，而且降低白树毒素在非靶细胞中的毒性。这些结果验证了“护送适体”可用于治疗表达独特抗原靶标的特定肿瘤的概念。
We have used RNA aptamer:gelonin conjugates to target and specifically destroy cells overexpressing the known cancer biomarker prostate-specific membrane antigen (PSMA). Aptamer:toxin conjugates have an IC50 of 27 nmol/L and display an increased potency of at least 600-fold relative to cells that do not express PSMA. The aptamer not only promotes uptake into target cells but also decreases the toxicity of gelonin in non-target cells. These results validate the notion that "escort aptamers" may be useful for the treatment of specific tumors expressing unique antigen targets.