COX-2 expression correlates with VEGF-C and lymph node metastases in patients with head and neck squamous cell carcinoma
COX-2 expression correlates with VEGF-C and lymph node metastases in patients with head and neck squamous cell carcinoma
复制标题
DOI：
10.1038/modpathol.3800244
复制
发表时间：
2005-01-01
期刊：
影响因子：
7.5
通讯作者：
Agnantis, NJ
中科院分区：
文献类型：
--
作者：
Kyzas, PA;Stefanou, D;Agnantis, NJ
最近的观察表明，环氧合酶-2（考克斯-2）通过上调血管内皮生长因子-C的表达而参与肿瘤淋巴管生成。目前尚不清楚这种机制是否也适用于头颈部鳞状细胞癌。我们对70例头颈部鳞状细胞癌进行了回顾性研究，以探讨考克斯-2免疫组化表达与血管内皮生长因子-C表达是否相关。我们还研究了这些分子的表达与这些患者的临床病理参数（特别是淋巴结状态）和预后的相关性。我们通过常规链霉亲和素-生物素过氧化物酶标记程序对福尔马林固定、石蜡包埋的组织切片进行免疫染色。在68个肿瘤样本中的30个（44%）中观察到环氧合酶-2表达增加，而在68个肿瘤样本中的26个（38%）中观察到血管内皮生长因子-C高表达。这两种蛋白的高表达与诊断时淋巴结转移的存在相关，当两种抗体都过表达时，观察到的相关性甚至更强（P
Recent observations suggest an implication of the cyclooxygenase-2 (COX-2) in tumor lymphangiogenesis through an upregulation of vascular endothelial growth factor-C expression. It is unknown whether this mechanism also acts in squamous cell carcinoma of the head and neck region. We performed a retrospective study of 70 patients with head and neck squamous cell carcinoma in order to investigate whether COX-2 immunohistochemical expression correlates with vascular endothelial growth factor-C expression. We also examined the association of the expression of these molecules with clinicopathologic parameters ( especially lymph node status) and outcome for these patients. We performed immunostaining on formalin-fixed, paraffin-embedded tissue sections by the routine streptavidin - biotin peroxidase labeled procedure. Increased cyclooxygenase-2 expression was observed in 30 of the 68 tumor samples (44%), while high vascular endothelial growth factor-C expression occurred in 26 of the 68 tumor samples (38%). High expression of the two proteins was correlated with the presence of lymph node metastasis at the time of diagnosis, and the observed association was even stronger when there was overexpression for both the antibodies ( P