Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans
Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans
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DOI：
10.7554/elife.57093
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发表时间：
2020-06-08
期刊：
影响因子：
7.7
通讯作者：
Flavell, Steven W.
中科院分区：
文献类型：
--
作者：
Cermak, Nathan;Yu, Stephanie K.;Flavell, Steven W.
动物行为通常被组织成持久的状态，这些状态协调地影响各种运动输出的产生，如进食、运动和梳理。然而，协调这些不同的运动程序的神经机制仍然知之甚少。在这里，我们研究了线虫C。elegans优雅are coupled耦合together一起across横过behavioral行为states状态.我们描述了一种新的成像平台，允许自动化，同时定量的每一个主要的C。elegans运动程序在几小时或几天内。对这些整体生物体行为特征的分析表明，运动程序随着动物切换行为状态而协调变化。利用遗传学、光遗传学和钙成像，我们确定了多巴胺在跨状态耦合运动和产卵中的新作用。这些结果为整个生物体中不同的运动程序是如何协调的提供了新的见解，并表明像多巴胺这样的神经调节剂可以以状态依赖的方式将运动回路耦合在一起。
Animal behaviors are commonly organized into long-lasting states that coordinately impact the generation of diverse motor outputs such as feeding, locomotion, and grooming. However, the neural mechanisms that coordinate these distinct motor programs remain poorly understood. Here, we examine how the distinct motor programs of the nematode C. elegans are coupled together across behavioral states. We describe a new imaging platform that permits automated, simultaneous quantification of each of the main C. elegans motor programs over hours or days. Analysis of these whole-organism behavioral profiles shows that the motor programs coordinately change as animals switch behavioral states. Utilizing genetics, optogenetics, and calcium imaging, we identify a new role for dopamine in coupling locomotion and egg-laying together across states. These results provide new insights into how the diverse motor programs throughout an organism are coordinated and suggest that neuromodulators like dopamine can couple motor circuits together in a state-dependent manner.