Anammox Bacterial Abundance, Activity, and Contribution in Riparian Sediments of the Pearl River Estuary
Anammox Bacterial Abundance, Activity, and Contribution in Riparian Sediments of the Pearl River Estuary
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珠江口河岸沉积物中厌氧氨氧化细菌的丰度、活性及其贡献
DOI：
10.1021/es3017446
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发表时间：
2012-08-21
影响因子：
11.4
通讯作者：
Yin, Chengqing
中科院分区：
文献类型：
--
作者：
Wang, Shanyun;Zhu, Guibing;Yin, Chengqing
基于我们对淡水交错带超过25年的研究以及之前对厌氧氨氧化的研究，我们提出了河流河岸带存在厌氧氨氧化热点的假设。在此，我们使用了一系列互补的方法，包括同位素配对技术、定量PCR分析以及16S rRNA和肼合成酶基因（hzsB）克隆文库，以记录河流河岸沉积物中厌氧氨氧化细菌高丰度区的时空证据，观测到的肼合成酶基因拷贝数在夏季为1.3 - 12×10⁶个/克，冬季为1.4 - 20×10⁸个/克，这是迄今为止在自然环境中所记录到的最高丰度。同时，检测到厌氧氨氧化细菌具有较高的生物多样性，以“Brocadia”和“Kuenenia”为主。然而，厌氧氨氧化细菌的高丰度与高活性以及对氮气产生的贡献并无关联。厌氧氨氧化活性在夏季为0.07 - 0.15纳摩尔氮/立方厘米·小时，冬季为1.0 - 2.6纳摩尔氮/立方厘米·小时，具有较高的时间异质性。古菌和细菌氨单加氧酶A（amoA）序列的检索表明，在冬季，硝化微生物可能是厌氧氨氧化细菌亚硝酸盐的主要来源，而在夏季，厌氧硝酸盐还原更有可能是主要来源。基于N - 15示踪技术，估计珠江河岸带沉积物中与厌氧氨氧化相关的总氮损失为0.67 - 9.62克氮/平方米·年，而反硝化作用的贡献为96.2 - 170.3克氮/平方米·年。
The hypothesis of an anammox hotspot in river riparian zones was put forward based on our investigation on freshwater ecotones for over 25 years and previous anammox research. Here we used a complementary array of methods including isotope-pairing technique, quantitative PCR assays, and 16S rRNA and hydrazine synthase gene (hzsB) clone libraries to document the spatiotemporal evidence for a high abundance zone of anammox bacteria in river riparian sediment with observed abundance of 1.3-12 x 10(6) (summer) and 1.4-20 x 10(8) (winter) hydrazine synthase gene copies g(-1), which is the highest abundance in natural environments recorded so far. Meanwhile high anammox bacterial biodiversity were detected with 'Brocadia' and 'Kuenenia' dominating. However, the high anammox bacterial abundances were not related with high activities and contributions for nitrogen gas generation. The anammox activities ranged from 0.07 to 0.15 nmol N cm(-3) h(-1) (summer) to 1.0-2.6 nmol N cm-3 h(-1) (winter) with high temporal heterogeneity. The retrieval of archaeal and bacterial amoA sequences indicated that nitrifying microbes might be the major source of nitrite for anammox bacteria in winter, while in summer the anaerobic nitrate reduction is more likely the main source. On the basis of N-15 tracing technology, it was estimated that a total loss of 0.67-9.62 g N m(-2) yr(-1) is linked to anammox in the riparian zone while denitrification contributed 96.2-170.3 g N m(-2) yr(-1) in Pearl River riparian sediments.