Expression of NF45 correlates with malignant grade in gliomas and plays a pivotal role in tumor growth
Expression of NF45 correlates with malignant grade in gliomas and plays a pivotal role in tumor growth
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NF45 的表达与神经胶质瘤的恶性程度相关，并在肿瘤生长中发挥关键作用
DOI：
10.1007/s13277-014-2310-5
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发表时间：
2014-10-01
期刊：
影响因子：
--
通讯作者：
Wang, Dongling
中科院分区：
文献类型：
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作者：
Huang, Qingfeng;He, Xiaojuan;Wang, Dongling
活化T细胞核因子45kda (NF45)，是一种与NF90相互作用调控基因表达的转录因子。它已被证明与各种人类恶性肿瘤的肿瘤增殖有关。然而，NF45在胶质瘤中的作用尚不清楚。我们的研究目的是探讨NF45在胶质瘤中表达与病理分级的关系，以及NF45对胶质瘤细胞增殖的影响。Western blot结果显示，与低级别胶质瘤组织相比，高级别胶质瘤组织中NF45的表达水平显著升高（P< 0.0001）。免疫组化结果也显示NF45在121例不同病理分级的切除胶质瘤中过表达，且与Ki-67相关。为了研究NF45在胶质瘤癌变中的作用，我们通过小干扰rna降低NF45的表达，结果显示NF45在体外克隆中抑制细胞增殖、阻滞细胞周期、减少。重要的是，我们发现SiNF45可以诱导p21的表达，降低增殖细胞核抗原（PCNA）和细胞周期蛋白e的表达，这些发现表明NF45在胶质瘤细胞的生长调节中起重要作用，提示NF45可能是恶性胶质瘤的病理分子标记和新的治疗靶点。
Nuclear factor of activated T cells 45 kDa (NF45), is a transcription factor that interacts with NF90 to regulate gene expression. It has been proved to be associated with tumor proliferation in various human malignancies. However, the role of NF45 in glioma is poorly understood. The aim of our study was to examine the relationship between NF45 expression and pathological grade in glioma and the impact of NF45 on the proliferation of glioma cells. Expression levels of NF45 are significantly elevated in high-grade human tissue samples compared with low-grade human glioma tissues samples (P< 0.0001) in Western blot analysis. The result of immunohistochemical also revealed that the expression of NF45 was overexpressed in 121 resected gliomas of different pathologic grades and associated with Ki-67. To investigate the role of NF45 in glioma carcinogenesis, we reduced the expression of NF45 by small interfering RNAs, and results showed suppression of cell proliferation, arrest of the cell cycle, and reduction in clone in vitro. Importantly, we show that SiNF45 can induce the expression of p21 and reduce the expression of proliferating cell nuclear antigen (PCNA) and cyclin E. These findings indicate that NF45 plays an important role in the growth regulation of glioma cells, suggesting that NF45 maybe a molecular marker for pathology and a novel therapeutic target for malignant glioma.