13C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research
13C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research
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DOI：
10.1021/ac900979e
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发表时间：
2009-08-01
影响因子：
7.4
通讯作者：
Willmitzer, Lothar
中科院分区：
文献类型：
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作者：
Giavalisco, Patrick;Koehl, Karin;Willmitzer, Lothar
代谢组学正迅速成为从疾病诊断到系统生物学的许多生命科学研究的一个组成部分。然而，在非靶向的基于LC-MS的代谢组学研究中，诸如生物信号与非生物信号的区分、有效的化合物注释和可靠的定量等许多问题仍然没有令人满意地解决。扩展我们以前的工作直接输液为基础的代谢组学，我们在这里描述了一个C-13同位素标记策略结合超高效液相色谱傅立叶变换离子回旋共振质谱法为基础的方法（UPLC-FTICR MS），它提供了一个技术平台，提供解决方案的一些上述问题。我们进一步证明，使用完全标记的代谢组不仅有利于高端质谱仪，如本研究中使用的质谱仪，而且还为其他基于质谱的代谢组学平台提供了相当大的改进。
Metabolomics is rapidly becoming an integral part of many life science studies ranging from disease diagnostics to systems biology. However, a number of problems such as the discrimination of biological from non-biological signals, efficient compound annotation, and reliable quantification are still not satisfactorily solved in untargeted LC-MS-based metabolomics research. Extending our previous work on direct infusion-based metabolomics, we here describe a C-13 isotope labeling strategy in combination with an Ultra Performance Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry-based approach (UPLC-FTICR MS) which provides a technological platform offering solutions to a number of the above-mentioned problems. We further demonstrate that the use of a fully labeled metabolome is not only beneficial for high end mass spectrometers, such as that used in this study but also provides a considerable improvement to every other mass spectrometry-based metabolomic platform.