Reply to Pancheva, Panchev, and Pancheva.
Reply to Pancheva, Panchev, and Pancheva.
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回复潘切瓦、潘切夫和潘切瓦。
DOI：
10.1152/japplphysiol.00449.2013
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发表时间：
2013
影响因子：
3.3
通讯作者：
Jue T.
中科院分区：
文献类型：
--
作者：
Masuda K;Yamada T;Jue T.
致编辑：我们感谢潘切娃博士及其同事的反馈和挑衅性评论(2)。根据我们最近的研究表明Mb与线粒体直接相互作用，Pancheva博士等人。提出了一种关于肌肉收缩过程中氧、三磷酸腺苷和聚合酶链式反应的即时代谢的理论：电流增强氧从血红素中的“分离”，机械收缩促进氧的释放并允许肌红蛋白作为过量的氧清除剂，温度升高降低了肌红蛋白的氧亲和力，二氧化碳在肌红蛋白中诱导玻尔效应。文献确实包含了对Pancheva等人的S理论(3)有价值的报道。许多研究已经研究了静电学如何帮助Mb区分配体结合，以及电场如何影响Mb的运动。然而，这些研究并没有声称生理上相关的电场可以改变血红素的Fe-O2共价键，并促进O2从血红素中“脱离”。事实上，许多研究都集中在静电对配体迁移的影响上，作者对扩散的限制性定义似乎排除了这一点(5)。尽管机械-化学耦合可以改变Mb的功能，但核磁共振研究表明，正如Pancheva等人的S理论(1)所暗示的那样，总体蛋白质效应的作用很小。血红素环境只对非常高的压力做出反应，以改变蛋白质的体积。机械-化学耦合的讨论如何导致支持Mb作为O2清除剂的作用的证据似乎还不清楚。研究已经表明，将温度从30摄氏度增加到35摄氏度将略微降低Mb的O2亲和力，
TO THE EDITOR: we appreciate the feedback and the provocative comments of Dr. Pancheva and coworkers (2). On the basis of our recent study showing a direct interaction of Mb with mitochondria, Dr. Pancheva et al. proposed a theory about the immediate metabolism of oxygen, ATP, and PCr during muscle contraction: electrical current enhances O2 “detachment” from heme, mechanical contraction facilitates O2 release and allows Mb to serve as an excessive O2 scavenger, elevated temperature decreases O2 affinity of Mb, and CO2 induces Bohr effect in Mb.The literature does contain a long history of reports that would cast invaluable perspectives on Pancheva et al.’s theory (3). Many studies have examined how electrostatics help Mb discriminate ligand binding and how the electric field could affect Mb movement. These studies, however, do not claim that a physiologically relevant electric field can alter the heme Fe-O2 covalent bond and facilitate the O2 “detachment” from the heme. In fact, many studies focus on the electrostatic effect on the ligand migration, which the authors’ restrictive definition of diffusion would appear to preclude (5). Although a mechanical-chemical coupling could alter the Mb function, NMR studies suggest a minimal role for an overall protein effect, as implied in Pancheva et al.’s theory (1). The heme environment responds only to very high pressure to change the protein volume. How the discussion of mechanical-chemical coupling leads to evidence supporting the role of Mb as an O2 scavenger seems unclear. Studies have already shown that increasing the temperature from 30 C to 35 C will decrease slightly the O2 affinity of Mb,
毛细管泵和二氧化碳的热控局部血流调节是提高训练肌床工作效率的基础。
影响因子：
3.3
作者：
Adelina V. Pancheva;Vladimir S. Panchev;M. Pancheva
通讯作者：
M. Pancheva
高碳酸血症对人体前臂血管的影响。
影响因子：
--
作者：
H. Kontos;D. Richardson;J. Patterson
通讯作者：
J. Patterson