Unusual plasmid DNA organization in an octopine crown gall tumor.
Unusual plasmid DNA organization in an octopine crown gall tumor.
复制标题
章鱼冠瘿瘤中异常的质粒 DNA 组织。
DOI：
10.1093/nar/13.2.459
复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Gordon,MP
中科院分区：
文献类型：
--
作者：
Kwok,WW;Nester,EW;Gordon,MP
本文研究了由根癌农杆菌15955菌株诱发的克隆烟草冠瘿瘤1595501。通过Southern转移和杂交技术对T-DNA组织的分子分析表明，1595501肿瘤具有约10个拷贝的TLDNA，其中5个是完整的TLDNA，而大多数章鱼肿瘤仅具有一到两个拷贝的完整的TLDNA。杂交研究和基因组克隆表明，T-DNA的某些片段发生了缺失。对1595501肿瘤细胞株的两个TLDNA左端和章鱼碱质粒相应区域进行了序列测定。各种克隆的T-DNA序列与Ti-质粒序列的比较表明，虽然存在与25个碱基对的直接重复的关联，但在T-DNA中没有发生与Ti-质粒序列的分歧的特定碱基对组。
A cloned tobacco crown gall tumor, 1595501, incited byA.tumefaciensstrain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TLDNA, five of which are complete TLDNA, whereas most octopine tumors have only one to two copies of complete TLDNA. Hybridization studies and genomic cloning indicated that some segments of the T-DNA have undergone deletions. One of the clones contained two copies of T-DNA which were inverted in orientation with respect to each other.Two left ends of TLDNA from the 1595501 tumor line and the corresponding region of the octopine plasmid were sequenced. Comparison of the various cloned T-DNA sequences with Ti-plasmid sequence indicated that while there is an association with a 25 base pair direct repeat, there is no specific set of base pairs in the T-DNA at which divergence from Ti-plasmid sequences occurred.