Molecular diversity of eukaryotes in municipal wastewater treatment processes as revealed by 18S rRNA gene analysis.
Molecular diversity of eukaryotes in municipal wastewater treatment processes as revealed by 18S rRNA gene analysis.
复制标题
如18S rRNA基因分析所示，在市政废水处理过程中真核生物的分子多样性。
DOI：
10.1264/jsme2.me14112
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发表时间：
2014
影响因子：
2.2
通讯作者：
Harada H
中科院分区：
文献类型：
--
作者：
Matsunaga K;Kubota K;Harada H
污水处理过程中涉及的真核生物群落已经通过形态学鉴定进行了研究，但还没有很好地利用分子方法进行表征。本研究通过构建18 S rRNA基因克隆文库对真核生物群落进行了表征。共843个克隆的系统发育隶属关系为：原生动物为泡孔动物、真菌、根瘤菌、裸藻动物、层生菌、变形虫和绿色植物，后生动物为轮虫、腹毛和线虫。60%的克隆与所描述的真核生物具有<97%的序列同一性，表明真核生物的多样性比以前认识到的更大。鉴定了与金寄蝇亲缘关系较近的一个核心OTU，其中有几个OTU被4-8个文库所共有。隐菌门中未培养的LKM 11菌系是污水处理过程中的优势菌。这项比较研究是进一步了解真核生物在污水处理过程中的多样性和作用的第一步。
Eukaryotic communities involved in sewage treatment processes have been investigated by morphological identification, but have not yet been well-characterized using molecular approaches. In the present study, eukaryotic communities were characterized by constructing 18S rRNA gene clone libraries. The phylogenetic affiliations of a total of 843 clones were Alveolata, Fungi, Rhizaria, Euglenozoa, Stramenopiles, Amoebozoa, and Viridiplantae as protozoans and Rotifera, Gastrotricha, and Nematoda as metazoans. Sixty percent of the clones had <97% sequence identity to described eukaryotes, indicating the greater diversity of eukaryotes than previously recognized. A core OTU closely related to Epistylis chrysemydis was identified, and several OTUs were shared by 4–8 libraries. Members of the uncultured lineage LKM11 in Cryptomycota were predominant fungi in sewage treatment processes. This comparative study represents an initial step in furthering understanding of the diversity and role of eukaryotes in sewage treatment processes.