Metabolism of xenobiotic compounds by enzymes in cell extracts of the fungus Cunninghamella elegans.
Metabolism of xenobiotic compounds by enzymes in cell extracts of the fungus Cunninghamella elegans.
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线虫真菌细胞提取物中的酶对异生化合物的代谢。
DOI：
10.1042/bj2050117
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Gibson,DT
中科院分区：
文献类型：
--
作者：
Wackett,LP;Gibson,DT
丝状真菌Cunninghamella elegans的细胞提取物具有环氧化物水解酶(EC 3.3.2.3)、谷胱甘肽S转移酶(EC 2.5.1.18)和UDP-葡萄糖醛酸基转移酶(EC 2.4.1.17)的活性。以对硝基环氧苯乙烷为底物测定了环氧化物水解酶的活性，结果表明环氧化物水解酶活性与从破碎的菌丝体中获得的100 000 g颗粒有关。以1-氯-2，4-二硝基苯和对硝基氯苄为底物，测定了谷胱甘肽S转移酶的活力。两种底物在不同提取物中的活性比率不同，热变性曲线也不同，表明存在两种或两种以上的谷胱甘肽S转移酶活性。以3-羟基苯并[a]芘为底物的UDP-葡萄糖醛酸基转移酶仅与破碎菌丝体制备的非沉淀组分有活性。
Cell extracts of the filamentous fungus Cunninghamella elegans contain epoxide hydrolase (EC 3.3.2.3), glutathione S-transferase (EC 2.5.1.18) and UDP-glucuronosyltransferase (EC 2.4.1.17) activities. Epoxide hydrolase activity was determined with p-nitrostyrene oxide as substrate and was shown to be associated with the 100 000 g pellet obtained from disrupted mycelia. Glutathione S-transferase activity was demonstrated with 1-chloro-2,4-dinitrobenzene and p-nitrobenzyl chloride as substrates. The presence of two or more glutathione S-transferase activities was indicated by different activity ratios for the two substrates in different extracts, and by distinct thermal denaturation curves. UDP-glucuronosyltransferase activity with 3-hydroxybenzo[a]pyrene as substrate was found only with the non-sedimentable fraction prepared from ruptured mycelia.