Embryonic and early postnatal cranial bone volume and tissue mineral density values for C57BL/6J laboratory mice.
Embryonic and early postnatal cranial bone volume and tissue mineral density values for C57BL/6J laboratory mice.
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C57 BL/6 J实验室小鼠的胚胎和出生后早期颅骨体积和组织矿物质密度值。
DOI：
10.1002/dvdy.458
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发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
基于人类和小鼠之间相对接近的遗传关系和发育途径的保守性，实验室小鼠通常用于颅面研究。由于遗传扰动和疾病状态可能具有局部效应，因此来自个体颅骨的数据对于解释实验测定是有价值的。我们采用高分辨率显微计算机断层扫描来表征C57 BL/6 J小鼠在胚胎第15.5天和E17.5天、出生当天（P0）和出生后第7天（P7）的颅骨，并提供个体骨体积和组织矿物质密度（TMD）的估计值。报告单个骨骼的平均体积和TMD值。胚胎期体积和TMD的显著差异可能反映了颅神经嵴衍生和膜内形成骨的早期矿化。虽然在胚胎时期，面部和穹窿的骨骼具有较高的TMD值，但在P7时，脑壳底部的骨骼具有显著较高的TMD值。这些关于颅骨体积和TMD的个体发生学数据可作为使用在C57 BL/6 J遗传背景上繁殖的小鼠进行未来研究的参考标准。我们的研究结果还强调了区分“对照”数据与“未受影响”的同窝小鼠的重要性，特别是当携带cre-重组酶基因的单拷贝时。胚胎发育期间颅神经嵴衍生和膜内形成骨的平均体积和密度较高。出生后早期发育过程中，脑壳底部骨骼的平均密度较高。关于颅骨体积和TMD的个体发育数据用作在C57 BL/6 J遗传背景上繁殖的小鼠的参考标准。
Laboratory mice are routinely used in craniofacial research based on the relatively close genetic relationship and conservation of developmental pathways between humans and mice. Since genetic perturbations and disease states may have localized effects, data from individual cranial bones are valuable for the interpretation of experimental assays. We employ high‐resolution microcomputed tomography to characterize cranial bones of C57BL/6J mice at embryonic day (E) 15.5 and E17.5, day of birth (P0), and postnatal day 7 (P7) and provide estimates of individual bone volume and tissue mineral density (TMD). Average volume and TMD values are reported for individual bones. Significant differences in volume and TMD during embryonic ages likely reflect early mineralization of cranial neural crest‐derived and intramembranously forming bones. Although bones of the face and vault had higher TMD values during embryonic ages, bones of the braincase floor had significantly higher TMD values by P7. These ontogenetic data on cranial bone volume and TMD serve as a reference standard for future studies using mice bred on a C57BL/6J genetic background. Our findings also highlight the importance of differentiating “control” data from mice that are presented as “unaffected” littermates, particularly when carrying a single copy of a cre‐recombinase gene. Higher average volume and density of cranial neural crest‐derived and intramembranously‐forming bones during embryonic development. Higher average density in bones of the braincase floor during early postnatal development. Ontogenetic data on cranial bone volume and TMD serve as a reference standard for mice bred on a C57BL/6J genetic background.
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影响因子：
3.7
作者：
Dorà NJ;Collinson JM;Hill RE;West JD
通讯作者：
West JD
DOI：
10.1002/wdev.227
发表时间：
2016-07
期刊：
Wiley interdisciplinary reviews. Developmental biology
影响因子：
--
作者：
Flaherty K;Singh N;Richtsmeier JT
通讯作者：
Richtsmeier JT
影响因子：
4.1
作者：
Beamer, WG;Donahue, LR;Baylink, DJ
通讯作者：
Baylink, DJ
影响因子：
4.1
作者：
Liu, Jin;Nam, Hwa Kyung;Campbell, Cassie;Gasque, Kellen Cristina da Silva;Millan, Jose Luis;Hatch, Nan E.
通讯作者：
Hatch, Nan E.
影响因子：
3
作者：
Davey, Rachel A.;Clarke, Michele V.;Zajac, Jeffrey D.
通讯作者：
Zajac, Jeffrey D.