Stimulation by Rad52 of yeast Rad51-mediated recombination
Stimulation by Rad52 of yeast Rad51-mediated recombination
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作者：
Shinohara, A;Ogawa, T
在酿酒酵母中，RAD 51和RAD 52基因参与重组和受损DNA的修复（1-3）。RAD 51基因是recA基因的结构和功能同源物（4，5），基因产物通过核蛋白丝形成参与链交换和单链DNA依赖性ATP水解（6-11）。RAD 52基因（12）在RAD 51介导的重组中是重要的（1-3）。该蛋白与Rad 51的结合（参考文献4，13）表明它们在重组中合作。Rad 51和Rad 52的同源物从酵母到人类都是保守的（14-16），这表明在重组过程中用于配对同源DNA分子的机制在真核生物中可能是通用的。在这里，我们表明，Rad 52蛋白刺激Rad 51反应，结合Rad 51是必要的，这种刺激作用。我们的结论是，这种结合是至关重要的重组，它有利于Rad 51核蛋白丝的形成。
In Saccharomyces cerevisiae, the RAD51 and RAD52 genes are involved in recombination and in repair of damaged DNA(1-3). The RAD51 gene is a structural and functional homologue of the recA gene(4,5) and the gene product participates in strand exchange and single-stranded-DNA-dependent ATP hydrolysis by means of nucleoprotein filament formation(6-11). The RAD52 gene(12) is important in RAD51-mediated recombination(1-3). Binding of this protein to Rad51 (refs 4, 13) suggests that they cooperate In recombination. Homologues of both Rad51 and Rad52 are conserved from yeast to humans(14-16), suggesting that the mechanisms used for pairing homologous DNA molecules during recombination may be universal in eukaryotes. Here we show that Rad52 protein stimulates Rad51 reactions and that binding to Rad51 is necessary for this stimulatory effect. We conclude that this binding is crucial in recombination and that it facilitates the formation of Rad51 nucleoprotein filaments.