Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry.
Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry.
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DOI：
10.1016/j.chroma.2013.08.016
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发表时间：
2013-09
期刊：
影响因子：
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通讯作者：
S. Lam;L. Tong;Bastien Reux;M. J. Lear;M. Wenk;G. Shui
中科院分区：
文献类型：
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作者：
S. Lam;L. Tong;Bastien Reux;M. J. Lear;M. Wenk;G. Shui
建立了一种快速、灵敏的人泪液脂质组蜡酯（WEs）定量分析方法。采用液相色谱法分离单个WE物种，采用特定多反应监测（MRM）扫描电喷雾电离质谱法检测。分别以棕榈酰棕榈酸酯和内部合成的蜡酯13c18:1（油酸-1,2,3,7,8,9,10- 13c7）C26:0作为内标，定量测定含有饱和脂肪酸和不饱和脂肪酸（FA）的WEs。检出限约为70 nmol/L。液相色谱-MRM检测WEs的线性范围约为3个数量级。对141个人泪液脂质组WE的定量分析表明，由FA18:1和FA16:1组成的物种分别占总WE的47.7%和24.0%（摩尔%），而人泪液WEs中的脂肪醇在17 ~ 32个碳之间，以C24、C25和C26为优势物种。
A rapid and sensitive method was developed for quantitative profiling of wax esters (WEs) in human tear lipidome. Individual WE species was separated by liquid chromatography and detected by electrospray ionisation mass spectrometry using specific multiple reaction monitoring (MRM) scanning. Palmitoyl palmitate and in-house synthesized wax esters13C18:1(oleic acid-1,2,3,7,8,9,10-13C7)C26:0 were used as internal standards for quantitation of WEs containing saturated and unsaturated fatty acids (FA), respectively. The limit of detection was approximately 70 nmol/L. The linearity range of the liquid chromatography (LC)-MRM detection for WEs was about three orders of magnitude. Quantitative analyses of 141 individual WE in the human tear lipidome demonstrated that species comprising FA18:1 and FA16:1 each accounted for 47.7% and 24.0% (molar%) of total WE, while fatty alcohols in WEs of human tears ranged from 17 carbons to 32 carbons with predominant species represented by C24, C25 and C26.