Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control
复制标题
DOI：
10.1038/s41467-019-13729-7
复制
发表时间：
2020-01-03
影响因子：
16.6
通讯作者：
Morita, Miyo Terao
中科院分区：
文献类型：
--
作者：
Furutani, Masahiko;Hirano, Yoshinori;Morita, Miyo Terao
在许多植物物种中，根保持相对于重力方向的特定生长角，称为向重力设定点角（GSA）。这有助于有效地获取水和养分。AtLAZY 1/LAZY 1-LIKE（LZY）基因通过调节生长素向重力方向流动参与拟南芥GSA调控。在这里，我们证明了RCC 1样结构域（RLD）蛋白，确定为LZY相互作用，是极性生长素运输的重要调节剂。我们发现LZY的CCL结构域与RLD的BRX结构域的相互作用对于LZY将RLD从细胞质募集到质膜是重要的。结构分析揭示了除了BRX结构域的结构之外，作为分子间β-折叠的相互作用模式。我们的研究结果提供了一个分子框架，其中重力信号首先出现在重力感应细胞中的极化LZY 3定位，然后是极性RLD 1定位和PIN 3重新定位以调节生长素流。
In many plant species, roots maintain specific growth angles relative to the direction of gravity, known as gravitropic set point angles (GSAs). These contribute to the efficient acquisition of water and nutrients. AtLAZY1/LAZY1-LIKE (LZY) genes are involved in GSA control by regulating auxin flow toward the direction of gravity in Arabidopsis. Here, we demonstrate that RCC1-like domain (RLD) proteins, identified as LZY interactors, are essential regulators of polar auxin transport. We show that interaction of the CCL domain of LZY with the BRX domain of RLD is important for the recruitment of RLD from the cytoplasm to the plasma membrane by LZY. A structural analysis reveals the mode of the interaction as an intermolecular beta-sheet in addition to the structure of the BRX domain. Our results offer a molecular framework in which gravity signal first emerges as polarized LZY3 localization in gravity-sensing cells, followed by polar RLD1 localization and PIN3 relocalization to modulate auxin flow.