Structure of the 70S ribosome complexed with mRNA and tRNA
Structure of the 70S ribosome complexed with mRNA and tRNA
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DOI：
10.1126/science.1131127
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发表时间：
2006-09-29
期刊：
影响因子：
56.9
通讯作者：
Ramakrishnan, V.
中科院分区：
文献类型：
--
作者：
Selmer, Maria;Dunham, Christine M.;Ramakrishnan, V.
细菌70 S核糖体的晶体结构精确到2.8埃分辨率，揭示了其与信使RNA（mRNA）和转移RNA（tRNA）相互作用的原子细节。金属离子稳定了mRNA中的扭结，该扭结划分了A和P位点之间的边界，这对于防止mRNA的滑动可能是重要的。金属离子也稳定亚基间的界面。与古细菌核糖体相比，E-site tRNA与50 S亚基的相互作用既有相似之处，也有不同之处。这种结构也使许多关于翻译的生物化学和遗传学数据合理化。
The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA ( mRNA) and transfer RNA ( tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.