Chemokine Receptor CXCR3 Expression in Inflammatory Bowel Disease
Chemokine Receptor CXCR3 Expression in Inflammatory Bowel Disease
复制标题
趋化因子受体 CXCR3 在炎症性肠病中的表达
DOI：
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发表时间：
2001
影响因子：
4.9
通讯作者：
A. T. Te Velde
中科院分区：
文献类型：
--
作者：
Yu;T. Hove;F. O. The;J. Frederik M. Slors;S. van Deventer;A. T. Te Velde
肠道固有层(LP)中的CD4+T淋巴细胞在炎症性肠病(IBD)的免疫反应中起核心作用。CXCR3是一种表达在活化T淋巴细胞上的趋化因子受体，是T辅助细胞(Th1)效应细胞向炎症部位募集的关键成分。为了确定CXCR3是否参与了IBD患者肠道T细胞的定位，我们研究了51例IBD患者和15例对照组切除标本中CD4+T细胞上CXCR3在LP和粘膜下层的表达。用5分制的半定量分析对阳性细胞进行显微镜评分。我们发现两组IBD患者LP中的CD4+T细胞、CXCR3+细胞和CD4+CXCR3+T细胞较非IBD对照组略有增加。此外，CD组粘膜下层的CD4+和CXCR3+细胞较对照组显著增加。CD和UC患者外周血中CD4+和CXCR3+的表达差异无统计学意义。用流式细胞术分析从IBD患者和对照患者的活检标本和外周血中分离出的CXCR3+细胞在CD4+T细胞群中的百分比。肠道和循环中不同组间的CD4+CXCR3+细胞百分率无差异。这些结果表明，CD4+CXCR3+T细胞迁移到正常和炎症的肠粘膜，表明在维持正常的肠道内环境平衡中起作用。CXCR3+细胞在CD患者黏膜下层的选择性表达也可能提示这些细胞在炎症中起作用。
CD4+ T lymphocytes in the lamina propria (LP) of the gut play a central role in the immune response in inflammatory bowel disease (IBD). CXCR3 is a chemokine receptor expressed on activated T lymphocytes, and a key component for the recruitment of T helper (Th1) effector cells to the site of inflammation. To determine if CXCR3 is involved in localization of T cells to the gut in IBD patients, we investigated the expression of CXCR3 on CD4+ T lymphocytes in the LP and in the submucosa of resection specimens from 51 IBD patients and 15 control patients. Positive cells were microscopically scored using a semiquantitative analysis on a five-point scale. We found that CD4+ T cells, CXCR3+ cells, and CD4+CXCR3+ T cells in the LP were slightly increased in both IBD groups compared with control non-IBD specimens. In addition, CD4+ and CXCR3+ cells in the submucosa were significant increased in the CD group compared with the control group. CD4+ and CXCR3+ expression was not statistically different between CD and UC. Flow cytometry was used to analyze the percentage of CXCR3+ cells within the CD4+ T-cell population isolated from biopsy specimens and peripheral blood from IBD patients and control patients. There was no difference in the percentage of CD4+CXCR3+ cells between the different groups in the gut as well as in the circulation. These results suggest that CD4+CXCR3+ T cells migrate to the normal and inflamed intestinal mucosa, indicating a role in maintaining normal gut homeostasis. The selective expression of CXCR3+ cells in the submucosa of CD patients might also indicate that these cells play a role in inflammation.
DOI：
10.1073/pnas.96.12.6873
发表时间：
1999-06-08
影响因子：
11.1
作者：
Balashov, KE;Rottman, JB;Hancock, WW
通讯作者：
Hancock, WW