A new dual -mode SERS and RRS aptasensor for detecting trace organic molecules based on gold nanocluster-doped covalent -organic framework catalyst
A new dual -mode SERS and RRS aptasensor for detecting trace organic molecules based on gold nanocluster-doped covalent -organic framework catalyst
复制标题
基于金纳米簇掺杂共价有机骨架催化剂的新型双模式SERS和RRS适体传感器检测痕量有机分子
DOI：
10.1016/j.snb.2020.128308
复制
发表时间：
2020-09-15
影响因子：
8.4
通讯作者：
Jiang, Zhiliang
中科院分区：
文献类型：
--
作者：
Yao, Dongmei;Li, Chongning;Jiang, Zhiliang
制备了一种新型金纳米簇掺杂共价有机骨架（AuCOF），并通过电子显微镜和分子光谱进行了表征。基于适配体介导的COF纳米催化放大信号策略，建立了表面增强拉曼散射/共振瑞利散射（SERS/RRS）双模式分析平台，用于检测超痕量小分子。研究了 AuCOFs 对 AgNO3-柠檬酸盐纳米反应的催化作用。形成的黄色银纳米颗粒（AgNPs）表现出很强的RRS和SERS效应。适配体 (Apt) 抑制 AuCOF 的催化作用，导致 RRS 和 SERS 信号减弱。添加目标有机分子如三磷酸腺苷（ATP）后，发生特异性适体反应，恢复AuCOFs的催化作用，从而产生更多的AgNPs，导致RRS和SERS信号线性增加。在此基础上，针对ATP、尿素、雌二醇等有机小分子建立了非弹性散射SERS、弹性散射RRS和SERS/RRS双模式生物传感器，与单模式散射方法相比灵敏度高、准确度好。
A new Au nanocluster-doped covalent organic framework (AuCOF) was prepared and characterized by electron microscopy and molecular spectroscopy. Based on aptamer-mediated COF nanocatalytic amplification signal strategy, a dual-mode analytical platform of surface-enhanced Raman scattering/resonance Rayleigh scattering (SERS/RRS) was established to detect ultatrace small molecules. The catalysis of AuCOFs on AgNO3-citrate nanoreaction was investigated. The formed yellow silver nanoparticles (AgNPs) exhibit strong RRS and SERS effect. The aptamer (Apt) suppressed the catalysis of AuCOFs to cause the RRS and SERS signals decreasing. Upon addition of target organic molecule such as adenosine triphosphate (ATP), the specific aptamer reaction occurred to restore the catalysis of AuCOFs, which will generate more AgNPs, and resulting in a linear increase in the signals of RRS and SERS. On this basis, inelastic scattering SERS, elastic scattering RRS and SERS/RRS dual-mode biosensors were established for small organic molecules such as ATP, urea, and estradiol, which is of high sensitivity and good accuracy compared to the single mode scattering method.