Visualizing the kinetics of tumor-cell clearance in living animals
Visualizing the kinetics of tumor-cell clearance in living animals
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DOI：
10.1073/pnas.96.21.12044
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发表时间：
1999-10-12
影响因子：
11.1
通讯作者：
Contag, CH
中科院分区：
文献类型：
--
作者：
Sweeney, TJ;Mailänder, V;Contag, CH
通过对活体动物肿瘤细胞的非侵入性检测，将加强对潜在的肿瘤治疗的评估。由于光通过哺乳动物组织传输，因此可以使用生物发光来监测（外部和定量）移植到免疫缺陷小鼠中的标记的人宫颈癌（HeLa）细胞的生长和消退。评价了用离体扩增的人T细胞衍生的效应细胞进行化疗和免疫治疗的功效。在没有治疗的情况下，动物显示信号强度随时间推移逐渐增加。用顺铂治疗的动物肿瘤信号显著减少; 5 '-氟尿嘧啶效果较差，环磷酰胺无效。免疫疗法在高效应细胞与靶细胞比率下显著降低信号，并且在较低比率下观察到显著降低。该模型系统允许灵敏的，定量的，实时的时空分析的肿瘤细胞生长的动态，并促进快速优化有效的治疗方案。
Evaluation of potential antineoplastic therapies would be enhanced by noninvasive detection of tumor cells in living animals. Because light is transmitted through mammalian tissues, it was possible to use bioluminescence to monitor (both externally and quantitatively) growth and regression of labeled human cervical carcinoma (HeLa) cells engrafted into immunodeficient mice. The efficacy of both chemotherapy and immunotherapeutic treatment with ex vivo expanded human T cell-derived effector cells was evaluated. In the absence of therapy, animals showed progressive increases in signal intensity over time. Animals treated with cisplatin had marked reductions in tumor signal; 5'-fluorouracil was less effective, and cyclophosphamide was ineffective. Immunotherapy dramatically reduced signals at high effector-to-target cell ratios, and significant decreases were observed with lower ratios. This model system allowed sensitive, quantitative, real-time spatiotemporal analyses of the dynamics of neoplastic cell growth and facilitated rapid optimization of effective treatment regimens.