Development of PLGA-Mannosamine Nanoparticles as Oral Protein Carriers
Development of PLGA-Mannosamine Nanoparticles as Oral Protein Carriers
复制标题
DOI：
10.1021/bm401141u
复制
发表时间：
2013-11-01
影响因子：
6.2
通讯作者：
Alonso, Maria J.
中科院分区：
文献类型：
--
作者：
Alonso-Sande, Maria;des Rieux, Anne;Alonso, Maria J.
在这里，我们报告了聚合物纳米颗粒的发展，由聚（丙交酯-共-乙交酯）（PLGA）与甘露糖胺（MN）化学修饰，旨在专门与肠粘膜相互作用，促进蛋白质的肠道运输。PLGA-MN纳米颗粒显示出纳米尺寸和负zeta电位，其低于PLGA纳米颗粒。这与通过X射线光电子能谱（XPS）获得的纳米颗粒表面上的MN基团的优先位置很好地相关。MN基团在聚合物链中的存在导致通过SEM观察到的不同的表面形态，增加了模型蛋白质的包封，并有助于在模拟肠液中稳定纳米颗粒。此外，MN修饰显著增强了纳米颗粒与人肠滤泡相关上皮细胞培养模型中上皮细胞的相互作用。总的来说，MN修饰显著改变了PLGA纳米颗粒的性质，使其更适合作为口服蛋白质递送的纳米载体。
Here we report the development of polymeric nanoparticles, made of poly(lactide-co-glycolide) (PLGA) chemically modified with mannosamine (MN), intended to specifically interact with the intestinal mucosa and facilitate the intestinal transport of proteins. PLGA-MN nanoparticles displayed nanometric size and a negative zeta potential, which was lower than that of the PLGA nanoparticles. This correlate well with the preferential location of the MN group on the nanoparticles surface obtained by X-ray photoelectron spectroscope (XPS). The presence of MN groups in the polymer chain led to a different surface morphology noted by SEM, an increase of the encapsulation of model proteins, and to help stabilizing the nanoparticles in simulated intestinal fluids. Furthermore, the MN modification significantly enhanced the nanoparticle's interaction with the epithelial cells in human intestinal follicle-associated epithelium cell culture model. Overall, the MN modification significantly modifies the properties of PLGA nanoparticles making them more suitable as nanocarriers for oral protein delivery.