INTERACTION BETWEEN THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-IIIB ENVELOPE GLYCOPROTEIN GP120 AND THE SURFACE-ANTIGEN CD4 - ROLE OF CARBOHYDRATE IN BINDING AND CELL-FUSION
INTERACTION BETWEEN THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-IIIB ENVELOPE GLYCOPROTEIN GP120 AND THE SURFACE-ANTIGEN CD4 - ROLE OF CARBOHYDRATE IN BINDING AND CELL-FUSION
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DOI：
10.1073/pnas.84.15.5424
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
BOLOGNESI, DP
中科院分区：
文献类型：
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作者：
MATTHEWS, TJ;WEINHOLD, KJ;BOLOGNESI, DP
与获得性免疫缺陷综合征相关的逆转录病毒与其淋巴细胞上的受体之间的相互作用代表了感染过程的初始步骤，也涉及多核巨细胞形成，这是病毒介导的细胞病理学的一种形式。逆转录病毒的外包膜糖蛋白已被鉴定为gp120，我们在这里证明纯化的gp120直接结合到表达CD4 （T4）表面抗原的细胞上，在空间上与OKT4A单克隆抗体识别的位点相关。gp120还能够暂时干扰病毒感染并阻断多核巨细胞形成的过程。然而，如果碳水化合物链通过酶处理从gp120中去除。CD4结合和细胞融合阻断减少了约50倍。讨论了这些结果在获得性免疫缺陷综合征的预防和干预方法方面的意义。
Interactions between retroviruses associated with acquired immunodeficiency syndrome and their receptors on lymphocytes represent the initial steps in the process of infection and are also involved in multinucleated giant cell formation, which is one form of virus-mediated cytopathology. The exterior envelope glycoprotein of the retrovirus has been identified as gp120, and we demonstrate here that purified gp120 binds directly to cells expressing the CD4 (T4) surface antigen at a site spatially related to that recognized by the OKT4A monoclonal antibody. The gp120 was also able to temporarily interfere with viral infection and to block the process of multinucleated giant cell formation. However, if the carbohydrate chains were removed from gp120 by enzymatic treatment. CD4 binding and blockade of cell fusion was reduced by about a factor of 50. The significance of these results in relation to preventive and interventive approaches for acquired immunodeficiency syndrome is discussed.