Resveratrol and Small Artery Compliance and Remodeling in the Spontaneously Hypertensive Rat
Resveratrol and Small Artery Compliance and Remodeling in the Spontaneously Hypertensive Rat
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DOI：
10.1038/ajh.2010.161
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发表时间：
2010-12-01
影响因子：
3.2
通讯作者：
Anderson, Hope D.
中科院分区：
文献类型：
--
作者：
Behbahani, John;Thandapilly, Sijo J.;Anderson, Hope D.
自发性高血压大鼠（SHR）的小动脉表现出异常的硬度和几何形状。本研究探讨了白藜芦醇，多酚类物质中发现的食物，如红葡萄，对小动脉在SHR.METHODSWistar京都（WKY）大鼠和SHR的影响与白藜芦醇（2.5 mg/kg/天）治疗10周。将肠系膜小动脉段（三级分支）安装在压力肌描记器中，并根据在递增的管腔内压力下测量的管腔和中膜尺寸计算血管几何形状和机械特性。尾袖体积描记法测量收缩期血压，SHR动脉与WKY动脉相比，顺应性增加，管壁成分硬度降低。虽然白藜芦醇并不能阻止血管壁成分硬度的降低，但它至少部分地减弱了SHR动脉顺应性的增加。相比之下，白藜芦醇增加了顺应性，降低了WKY动脉壁成分的硬度。SHR动脉表现出重塑，包括狭窄的管腔，增厚的中膜宽度，并增加中膜管腔比。白藜芦醇部分减弱了重塑过程，也取消了夸张的ERK信号和增殖细胞核抗原（增殖的标志物）在SHR动脉的表达。后者的作用可能与白藜芦醇减轻SHR氧化应激和增强蛋白激酶G（PKG）活性的能力有关。在20周龄的SHR血压升高是不受白藜芦醇。CONCLUSIONSThe白藜芦醇的能力，以限制SHR动脉顺应性的增加可能与抑制作用重塑和促生长ERK信号，而不是血压或动脉壁组件刚度。
BACKGROUNDSmall arteries from the spontaneously hypertensive rat (SHR) exhibit abnormal stiffness and geometry. This study investigated the effects of resveratrol, a polyphenol found in foods such as red grapes, on small arteries in SHR.METHODSWistar Kyoto (WKY) rats and SHR were treated with resveratrol (2.5 mg/kg/day) for 10 weeks. Mesenteric small artery segments (third-order branches) were mounted in a pressure myograph, and vascular geometry and mechanical properties were calculated from lumen and media dimensions measured at incremental intraluminal pressures. Systolic blood pressure was measured by tail-cuff plethysmography.RESULTSIncreased compliance and reduced wall component stiffness were observed in SHR arteries vs. WKY arteries. Though resveratrol did not prevent lowering of wall component stiffness, it did attenuate, at least in part, the increased compliance of SHR arteries. In contrast, resveratrol increased compliance and reduced wall component stiffness in WKY arteries. SHR arteries exhibited remodeling that consisted of narrowed lumens, thickened media widths, and augmented media-to-lumen ratios. Resveratrol partially attenuated the remodeling process and also abolished exaggerated ERK signaling and expression of proliferating cell nuclear antigen (a marker of proliferation) in SHR arteries. The latter effects might be related to the ability of resveratrol to alleviate oxidative stress in SHR and enhance protein kinase G (PKG) activity. Elevated blood pressure in 20-week-old SHR was unaffected by resveratrol.CONCLUSIONSThe ability of resveratrol to limit the increase in compliance of SHR arteries is likely related to inhibitory effects on remodeling and pro-growth ERK signaling rather than blood pressure or arterial wall component stiffness.