Effects of Yinchenhao Tang and related decoctions on DMN-induced cirrhosis/fibrosis in rats.
Effects of Yinchenhao Tang and related decoctions on DMN-induced cirrhosis/fibrosis in rats.
复制标题
茵陈蒿汤及其相关汤剂对 DMN 所致大鼠肝硬化/纤维化的影响。
DOI：
10.1186/1749-8546-3-1
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发表时间：
2008-01-31
期刊：
影响因子：
4.9
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Liu C;Sun M;Wang L;Wang G;Chen G;Liu C;Liu P
采用茵陈蒿汤、下瘀血汤、黄芪汤、一贯煎、小柴胡汤等治疗肝硬化。本实验观察了这些中药对二甲基亚硝胺（DMN）诱导的大鼠肝纤维化的影响。将DMN溶液（0.5%）注射至大鼠，每周连续三天，持续四周。第3周开始，将大鼠随机分为4周DMN对照组、YCHT组、XYXT组、HQT组、YGJ组、XCHT组和溶媒组。两组均每日口服特定的预防措施，持续两周。溶媒组大鼠仅用水经口给药。DMN中毒大鼠在第2周和第4周出现肝纤维化和肝硬化。与正常大鼠相比，模型组大鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）活性、总胆红素酸（TBA）水平及肝组织羟脯氨酸（Hyp）含量均显著升高。但血清白蛋白（Alb）水平显著降低。与DMN 4周组相比，YCHT组大鼠肝脏病理形态和功能明显改善，Hyp含量明显降低，仅1只大鼠发生肝硬化，肝硬化发生率仅为8.3%。另一方面，其他预防措施没有显示出显著的效果。YCHT抑制α-SMA的激活，包括其基因表达为mRNA和蛋白。在5种中药复方中，以YCHT对DMN诱导的大鼠肝硬化/纤维化的治疗作用最显著。
Chinese medicine decoctions such as Yinchenhao Tang (YCHT), Xiayuxue Tang (XYXT), Huangqi Tang (HQT), Yiguan Jian (YGJ) and Xiaochaihu Tang (XCHT)) were used to treat liver cirrhosis. The present study evaluates the effects of these decoctions on fibrosis in rats induced by dimethylnitrosamine (DMN). DMN solution (0.5%) was injected to rats for three consecutive days per week for four weeks. At the beginning of week 3, rats were randomly divided into 4-week DMN control group, YCHT, XYXT, HQT, YGJ, XCHT and vehicle groups. Each group was orally administered with specific decoctions daily for two weeks. Rats in the vehicle group were orally administered with only water. Liver fibrosis and cirrhosis were observed in weeks 2 and 4 in DMN-intoxicated rats. Compared with normal rats, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) activities and level of total bilirubin acid (TBA) in serum and content of Hydroxyproline (Hyp) in liver tissue of model group rats rose significantly. However, the albumin (Alb) level in serum decreased significantly. Compared with the 4-week DMN group, the pathological conditions and functions of the liver in the YCHT group improved significantly, and the content of Hyp decreased remarkably: only one rat in this group developed liver cirrhosis and the ratio of cirrhosis was only 8.3%. On the other hand, the other decoctions did not show remarkable effects. YCHT inhibited α-SMA activation, including its gene expression into mRNA and protein. Among the five Chinese medicine decoctions, YCHT exerted the most significant therapeutic effects on DMN-induced cirrhosis/fibrosis in rats.