Quantitative DIC microscopy using an off-axis self-interference approach.
Quantitative DIC microscopy using an off-axis self-interference approach.
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DOI：
10.1364/ol.35.002370
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发表时间：
2010-07-15
期刊：
影响因子：
3.6
通讯作者：
Feld MS
中科院分区：
文献类型：
--
作者：
Fu D;Oh S;Choi W;Yamauchi T;Dorn A;Yaqoob Z;Dasari RR;Feld MS
传统的Normarski微分干涉对比（DIC）显微镜是一种非常强大的方法，用于成像非染色的生物样品。然而，其主要局限性之一是成像的非定量性质。为了克服这个问题，我们开发了一种定量DIC显微镜方法的基础上离轴样品自干涉。该方法利用数字全息算法在正交方向上获得定量的相位梯度，通过对相位梯度的螺旋积分得到定量的相位图像。该方法在任何标准显微镜上实现起来都很简单，对偏振光学器件没有严格的要求。光学切片可以通过放大照明数值孔径（NA）来获得。
Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging non-stained biological samples. However, one of its major limitations is the non-quantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample self-interference. The digital holography algorithm is applied to obtain quantitative phase gradients in orthogonal directions, which leads to quantitative phase image through a spiral integration of the phase gradients. This method is practically simple to implement on any standard microscope without stringent requirement on polarization optics. Optical sectioning can be obtained through enlarged illumination numerical aperture (NA).