AGROBACTERIUM-MEDIATED TRANSFORMATION OF ALMOND LEAF PIECES
AGROBACTERIUM-MEDIATED TRANSFORMATION OF ALMOND LEAF PIECES
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DOI：
10.1007/bf00232026
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发表时间：
1995-02-01
影响因子：
6.2
通讯作者：
DAMIANO, C
中科院分区：
文献类型：
--
作者：
ARCHILLETTI, T;LAURI, P;DAMIANO, C
A protocol for Agrobacterium-mediated transformation of leaf pieces from two almond cultivars has been developed. This protocol gave a transformation percentage of 48.6 for the cultivar MN51 and 27.9 for the cultivar 'Supernova'. The Agrobacterium tumefaciens strain LBA 4404 carrying a binary vector plasmid pBinGUSint has been used. A critical step for optimal transformation was the pre-culture period of leaf pieces prior selection on medium containing kanamycin. Both cultivars showed an almost doubled transformation percentage when the selection was started at day 6 compared to day 0. The presence of neomycin phosphotransferase II and beta-glucuronidase genes carried by pBinGUSint was confirmed in transformed explants both by Southern blotting analysis and enzyme activity assays.
