A Novel Surgical Approach for the Reconstruction of Critical-Size Mandibular Defects Using Calcium Sulphate/Hydroxyapatite Cement, BMP-7 and Mesenchymal Stem Cells-Histological Assessment
A Novel Surgical Approach for the Reconstruction of Critical-Size Mandibular Defects Using Calcium Sulphate/Hydroxyapatite Cement, BMP-7 and Mesenchymal Stem Cells-Histological Assessment
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DOI：
10.1166/jbt.2016.1412
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发表时间：
2016-01-01
影响因子：
0.1
通讯作者：
McMahon, Jeremy
中科院分区：
文献类型：
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作者：
Alfotawi, Randa;Ayoub, Ashraf F.;McMahon, Jeremy
一种新的结构已被开发出来，以诱导骨形成的蒂肌瓣。在10只家兔的下颌骨中创建临界尺寸缺损（20 x 15 mm（2））。使咬肌适合于填充手术缺损，并将硫酸钙/羟基磷灰石水泥（CERAMENTTM垂直杆脊柱支撑）、BMP-7和兔间充质基质细胞（rMSC）的组合注射到肌肉组织中。术后3个月评价骨再生情况。尽管新骨在整个肌肉和骨水泥残留物周围的结缔组织内形成，但骨形成的有限区域在解剖学上桥接了缺损。与对侧（非手术）侧相比，颊舌方向的骨更厚。定量组织形态学评估显示，平均骨表面积为21.2 +/- 6.0 mm（2），显著大于对侧非手术对照侧。残余骨水泥和软组织或间隙的计算量分别为20 +/- 12%和41 +/-10%。平均矿物沉积速率（MAR）为1.92 μ m/天。研究结果表明，使用局部肌瓣的显着潜力，可注射的生物水泥加载BMP和种植与rMSCs诱导骨形成的骨缺损重建。
A novel construct has been developed to induce bone formation within a pedicle muscle flap. A critical size defect (20 x 15 mm(2)) was created in the mandible of ten rabbits. The masseter muscle was adapted to fill the surgical defect and a combination of calcium sulphate/hydroxyapatite cement (CERAMENT (TM) vertical bar SPINE SUPPORT), BMP-7 and rabbits' mesenchymal stromal cells (rMSCs) was injected into the muscle tissue. Bone regeneration was evaluated 3 months after surgery. Limited areas of bone formation anatomically bridged the defect, despite the new bone forming throughout the muscle and within the connective tissue surrounding the remnants of the cement. The bone was thicker in the bucco-lingual direction compared to the contra lateral (non-operated) side. Quantitative histomorphometry assessment showed that the average bone surface area was 21.2 +/- 6.0 mm(2), this was significantly greater than that of the contra-lateral non-operated control side. The calculated amounts of residual cement and soft tissue or spaces were 20 +/- 12% and 41 +/- 10%, respectively. The average mineral apposition rate (MAR) was 1.92 mu m/day. The findings demonstrated the remarkable potential of the use of local muscle flaps for injectable bio-cement loaded with BMP and seeded with rMSCs to induce bone formation for the reconstruction of bony defects.