Plk1 is negatively regulated by RNF8.
Plk1 is negatively regulated by RNF8.
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DOI：
10.1016/j.bbrc.2011.05.104
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发表时间：
2011-06
影响因子：
3.1
通讯作者：
Takashi Yoshioka;M. Kimura;M. Saio;S. Era;Y. Okano
中科院分区：
文献类型：
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作者：
Takashi Yoshioka;M. Kimura;M. Saio;S. Era;Y. Okano
RNF 8是具有N-末端叉头相关（FHA）结构域和C-末端环指（RF）结构域的核蛋白。RNF 8的缺失导致细胞生长抑制和细胞周期停滞在S期和G2/M期。此外，在RNF 8耗尽的细胞中经常观察到细胞死亡。延时显微镜分析显示，细胞在有丝分裂期和分裂间期死亡。为了阐明RNF 8在M期的功能，检测RNF 8缺失的细胞中Plk 1的含量。RNF 8的量随时间的推移而减少，而Plk 1的量随着RNF 8 siRNA的转染而增加。比较了不同细胞系中RNF 8和Plk 1蛋白的含量。RNF 8在正常细胞系中的表达明显高于许多癌细胞系。相反，Plk 1在正常细胞系中低于癌细胞系。这些结果表明，RNF 8在许多癌细胞中下调，并与Plk 1呈负相关。
RNF8 is a nuclear protein having an N-terminal forkhead-associated (FHA) domain and a C-terminal RING-finger (RF) domain. Depletion of RNF8 caused cell growth inhibition and cell cycle arrest at not only S but also G2/M phases. In addition, cell death was frequently observed in RNF8-depleted cells. Analyses of time-lapse microscopy revealed that the cells died in mitosis and interphase. To elucidate the RNF8 function in M phase, the Plk1 content in RNF8-depleted cells was examined. The amount of RNF8 decreased time-dependently, whereas Plk1 reciprocally increased by transfection of RNF8 siRNA. Protein contents of RNF8 and Plk1 among various cell lines were also compared. RNF8 in normal cell lines was much higher than that in many cancer cell lines. Conversely, Plk1 in normal cell lines was lower than in cancer cell lines. These results suggest that RNF8 is downregulated in many cancer cells and inversely correlated with Plk1.