TARGET ENZYME-BASED RESISTANCE TO ACETYL-COENZYME-A CARBOXYLASE INHIBITORS IN ELEUSINE-INDICA
TARGET ENZYME-BASED RESISTANCE TO ACETYL-COENZYME-A CARBOXYLASE INHIBITORS IN ELEUSINE-INDICA
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DOI：
10.1006/pest.1995.1013
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发表时间：
1995-02-01
影响因子：
4.7
通讯作者：
MARSHALL, G
中科院分区：
文献类型：
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作者：
LEACH, GE;DEVINE, MD;MARSHALL, G
牛筋草的一个生物型。Gaertn.来自马来西亚的一个具有4-5年内每年施用两次或三次精吡氟禾草灵的田间历史的群体，先前发现在田间条件下与易感群体相比显示出100倍的除草剂抗性。该生物型对其他芳氧基苯氧基丙酸酯和环己二酮除草剂具有交叉抗性。以前的研究表明，没有证据表明R和S生物型之间的氟吡禾灵的吸收，转运或代谢差异。氟吡禾灵酸减少[C-14]乙酸盐掺入到S生物型叶盘的脂质部分，但不是R生物型。来自S生物型的乙酰辅酶A羧化酶（ACCase）对氟吡禾灵、精恶唑禾草灵、稀禾啶和烯草酮敏感，I-50值范围为1.0至5.6 μ M。相反，来自R生物型的ACCase对氟吡禾灵、精恶唑禾草灵和稀禾啶的敏感性低得多，I-50值分别>500、25和77 μ M。来自R生物型的ACCase仅显示出对烯草酮的低水平抗性，I-50为6.6 μ M。整个植物和ACCase对各种ACCase抑制剂的敏感性之间的密切相关性表明，该生物型中的除草剂抗性由除草剂靶位点ACCase的突变赋予。(C)出版社：Academic Press
A biotype of Eleusine indica L. Gaertn. from Malaysia, which had a field history of two or three applications of fluazifop-p-butyl per year over 4-5 years, was previously found to show 100-fold resistance to the herbicide under field conditions compared with a susceptible population. This biotype is cross-resistant to other aryloxyphenoxypropanoate and cyclohexanedione herbicides. Previous research has shown no evidence for differential uptake, translocation, or metabolism of fluazifop-butyl between the R and S biotypes. Fluazifop acid reduced [C-14]acetate incorporation into the lipid fraction of leaf disks of the S biotype but not of the R biotype. Acetyl coenzyme A carboxylase (ACCase) from the S biotype was sensitive to fluazifop, fenoxaprop, sethoxydim, and clethodim, with I-50 values ranging from 1.0 to 5.6 mu M. In contrast, ACCase from the R biotype was much less sensitive to fluazifop, fenoxaprop, and sethoxydim, with I-50 values of >500, 25, and 77 mu M, respectively. ACCase from the R biotype showed only a low level of resistance to clethodim, with an I-50 of 6.6 mu M. The close correlation between the whole plant and ACCase sensitivities to various ACCase inhibitors suggests that herbicide resistance in this biotype is conferred by a mutation to the herbicide target site, ACCase. (C) 1995 Academic Press, Inc.