Transstadial and intrastadial experimental transmission of Ehrlichia canis by male Rhipicephalus sanguineus
Transstadial and intrastadial experimental transmission of Ehrlichia canis by male Rhipicephalus sanguineus
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DOI：
10.1016/j.vetpar.2005.04.030
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发表时间：
2005-07-15
影响因子：
2.6
通讯作者：
Stich, RW
中科院分区：
文献类型：
--
作者：
Bremer, WG;Schaefer, JJ;Stich, RW
不同发育阶段立克次体病原体的获取和传播具有重要的流行病学意义。这项研究的目的是确定在没有雌性扁虱的情况下，雄性血缘里氏杆菌是否能够实验性地获取和传播犬埃立克氏菌。进行了两个试验，其中若虫和雄性血吸虫同时在同一受感染的供体宿主上采集，并通过经口或经口内暴露的雄性硬蜱喂养不同的无病原体的狗作为传播测试。以P30为基础的一步聚合酶链式反应方法用于检测犬类和硬体宿主在硬体饲养前后犬棘球绦虫的感染情况。在雄蚤体内或经肠道传代后，均可检出犬立克次体，经传食后两组均可检出犬立克次体，两组均可将立克次体传播给敏感犬。与静脉接种携带者血液相比，通过扁虱喂养感染犬的临床症状较轻，抗体效价较低，但需要进一步调查以了解导致这一观察结果的机制。这些结果表明，雄性血吸虫可以进行多次摄食，并且在没有雌性扁虱的情况下，它们可以获取和传播犬内棘球绦虫。在自然和实验条件下，在脊椎动物宿主之间传播犬埃希菌和相关病原体的过程中，这一扁虱发育阶段可能是重要的。(C)2005 Elsevier B.V.保留所有权利。
The acquisition and transmission of rickettsial pathogens by different tick developmental stages has important epidemiological implications. The purpose of this study was to determine if male Rhipicephalus sanguineus can experimentally acquire and transmit Ehrlichia canis in the absence of female ticks. Two trials were performed where nymphal and male R. sanguineus were simultaneously acquisition fed on the same infected donor hosts, and transstadially or intrastadially exposed male ticks were fed on separate pathogen-free dogs as a test for transmission. A single-step p30-based PCR assay was used to test canine and tick hosts for E. canis infections before and after tick feeding. E. canis was detected after either intrastadial or transstadial passage in male ticks, the organism remained detectable in both tick groups after transmission feeding, and both tick groups transmitted the rickettsia to susceptible dogs. Infection of dogs via tick feeding resulted in milder clinical signs and lower antibody titers than intravenous inoculation of carrier blood, but further investigation is needed to understand the mechanisms responsible for this observation. These results demonstrate that male R. sanguineus can take multiple feedings, and that they can both acquire and transmit E. canis in the absence of female ticks. This tick development stage could be important in transmission of E. canis, and perhaps related pathogens, between vertebrate hosts under natural and experimental conditions. (c) 2005 Elsevier B.V. All rights reserved.