The soya isoflavone content of rat diet can increase anxiety and stress hormone release in the male rat
The soya isoflavone content of rat diet can increase anxiety and stress hormone release in the male rat
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DOI：
10.1007/s00213-002-1369-7
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发表时间：
2003-04-01
影响因子：
3.4
通讯作者：
File, SE
中科院分区：
文献类型：
--
作者：
Hartley, DE;Edwards, JE;File, SE
依据：大多数商业啮齿动物饲料都是用大豆蛋白配制的，因此含有大豆异黄酮。异黄酮形成植物雌激素的主要类别之一，并且已发现对中枢神经系统发挥雌激素和抗雌激素作用。影响不仅限于生殖行为，还包括对学习和焦虑的影响以及对下丘脑-脑垂体轴的作用。因此，饮食中的大豆含量可能对大脑和行为产生显著影响，并且可能是实验室间变异性的重要来源。目的：为了确定是否行为在两个动物测试的焦虑，和应激激素的生产，将不同的大鼠之间的饮食是免费的大豆黄酮和其他植物雌激素（isofree）和那些。喂食含有150 μ g/g染料木黄酮和大豆黄酮（ISO-150）的饮食。这种控制饮食的维生素C浓度与我们机构常规使用的维持饮食中的维生素C浓度相似。研究方法：将雄性大鼠随机分配至不含iso和iso-150饲料组，并记录其体重、摄食量和饮水量，持续14天。然后，他们保持相同的饮食，但单独圈养4天，然后在社交互动和高架十字迷宫测试焦虑。皮质酮浓度在两个饮食组的基础条件下，并在两个测试的焦虑的压力。加压素和催产素的浓度测定后，短暂的处理压力。结果各组在食物或水摄入量、体重或催产素浓度方面没有差异。与喂食无iso饮食的大鼠相比，喂食iso-150饮食的大鼠在积极的社会互动中花费的时间显著减少，并且进入十字迷宫开放臂的百分比显著降低，这表明在两种动物试验中均具有致焦虑作用。各组的基础皮质酮浓度没有差异，但iso-150组有显着升高的应激诱导的皮质酮浓度。应激诱导的血浆血管加压素浓度也显着升高，在iso-150饮食组与isofree大鼠相比。结论：大鼠饮食中的大豆蛋白含量可能导致焦虑行为指标和应激激素的重大变化。这些变化与给药后观察到的变化一样引人注目，可能成为实验室之间变异的重要来源。
Rationale: Most commercial rodent diets are formulated with soya protein and therefore contain soya isoflavones. Isoflavones form one of the main classes of phytoestrogens and have been found to exert both oestrogenic and anti-oestrogenic effects on the central nervous system. The effects have not been limited to reproductive behaviour, but include effects on learning and anxiety and actions on the hypothalamo-pituitary axis. It is therefore possible that the soya content of diet could have significant effects on brain and behaviour and be an important source of between-laboratory variability. Objectives: To determine whether behaviour in two animal tests of anxiety, and stress hormone production, would differ between rats that were fed a diet which was free of soya isoflavones and other phytoestrogens (isofree) and those. that were fed a diet which contained 150 mug/g of the isoflavones genistein and daidzein (iso-150). This controlled diet has an isoflavone concentration similar to that in the maintenance diet routinely used in our institution. Methods: Male rats were randomly allocated to the iso-free and iso-150 diets and their body weights and food and water consumption were recorded for 14 days. They were then maintained on the same diets, but housed singly for 4 days, before testing in the social interaction and elevated plus-maze tests of anxiety. Corticosterone concentrations in both dietary groups were determined under basal conditions and after the stress of the two tests of anxiety. Vasopressin and oxytocin concentrations were determined after brief handling stress. Results The groups did not differ in food or water intake, body weight or oxytocin concentrations. Compared with the rats fed the iso-free diet, the rats fed the iso-150 diet spent significantly less time in active social interaction and made a significantly lower percentage of entries onto the open arms of the plus-maze, indicating anxiogenic effects in both animal tests. The groups did not differ in their basal corticosterone concentrations, but the iso-150 group had significantly elevated stress-induced corticosterone concentrations. Stress-induced plasma vasopressin concentrations were also significantly elevated in the iso-150 diet group compared with the isofree rats. Conclusions: Major changes in behavioural measures of anxiety and in stress hormones can result from the soya isoflavone content of rat diet. These changes are as striking as those seen following drug administration and could form an important source of variation between laboratories.