Dielectrophoretic Technique for Measurement of Chemical and Biological Interactions
Dielectrophoretic Technique for Measurement of Chemical and Biological Interactions
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DOI：
10.1021/ac901211b
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发表时间：
2009-09-15
影响因子：
7.4
通讯作者：
Lee, Sang Woo
中科院分区：
文献类型：
--
作者：
Baek, Sang Hyun;Chang, Woo-Jin;Lee, Sang Woo
我们提出了一种新的介电泳法，可以用来表征微流控器件内部的分子相互作用。我们的方法允许最初停留在功能化表面上的功能化珠子通过作用在珠子上的介电泳力从表面上拉出。因此，可以定量地检测表面分子与珠子之间的相互作用。我们报告了详细的实验结果，并用模型验证了结果，表明该技术可以用来测量各种实验条件下的分子间相互作用力。
We present a novel dielectrophoretic technique that can be used to characterize molecular interactions inside a microfluidic device. Our approach allows functionalized beads which are initially at rest on a functionalized surface to be pulled away from the surface by the dielectrophoretic force acting on the beads. As a result, the interaction between the molecules on the surface and the beads can be quantitatively examined. We report detailed experimental result and validate the results with a model to show that the technique can be used to measure forces of interaction between molecules under various experimental conditions.