In vivo absorption, scattering, and physiologic properties of 58 malignant breast tumors determined by broadband diffuse optical spectroscopy
In vivo absorption, scattering, and physiologic properties of 58 malignant breast tumors determined by broadband diffuse optical spectroscopy
复制标题
DOI：
10.1117/1.2337546
复制
发表时间：
2006-07-01
影响因子：
3.5
通讯作者：
Tromberg, Bruce J.
中科院分区：
文献类型：
--
作者：
Cerussi, Albert;Shah, Natasha;Tromberg, Bruce J.
漫反射光学成像(DOI)对于乳房X光摄影致密的女性来说可能是一种有益的诊断方法。为了评估DOI的实用性，我们正在开发宽带漫反射光谱(DOS)来表征乳腺癌患者光信号的功能来源。宽带DOS结合了多频强度调制和连续波近红外光，以量化组织从650到1000 nm的吸收和散射光谱。从吸收光谱和散射光谱得出的内在生理特性(氧合和脱氧血红蛋白、水、脂肪和散射功率)值提供了有关乳房生理学的详细信息。我们介绍了使用无创、手持、宽带DOS探头对58例II/III期乳腺癌的临床研究结果。平均而言，每个受试者在肿瘤和对侧正常乳房上扫描8个位置。在所有受试者中，在没有患者年龄或肿瘤大小/类型分层的情况下，恶性组织和正常组织的内在生理特性在统计学上有显著差异。与正常乳腺组织相比，含有恶性肿瘤的乳腺组织的脂肪含量降低(接近20%)，水分、脱氧和氧合血红蛋白增加(均为50%)。肿瘤引起的功能紊乱明显大于正常组织中的功能变异。组织光学指数(TOI)来自固有的生理特性，在恶性肿瘤和固有组织特性之间产生平均两倍的对比度差异。我们的结果表明，固有的光信号可以受到恶性转化的功能扰动、细胞代谢、细胞外基质组成和血管生成的影响。我们的研究结果进一步强调了宽带测量和患者年龄分层在乳腺癌DOI中的重要性。(C)2006年光学仪器工程师学会。
Diffuse optical imaging (DOI ) may be a beneficial diagnostic method for women with mammographically dense breast tissue. In order to evaluate the utility of DOI, we are developing broadband diffuse optical spectroscopy (DOS) to characterize the functional origins of optical signals in breast cancer patients. Broadband DOS combines multifrequency intensity-modulated and continuous-wave near-infrared light to quantify tissue absorption and scattering spectra from 650 to 1000 nm. Values of intrinsic physiological properties (oxy-and deoxy-hemoglobin, water, lipid, and scatter power) derived from absorption and scattering spectra provide detailed information on breast physiology. We present the results of clinical studies of 58 stage II/III malignant breast tumors using a noninvasive, handheld, broadband DOS probe. On average, eight positions were scanned over tumor and contralateral normal breast for each subject. Intrinsic physiological properties were statistically significantly different for malignant vs. normal tissues for all subjects, without patient age or tumor size/type stratification. Breast tissues containing malignant tumors displayed reduced lipid content (similar to 20%) and increased water, deoxy-, and oxy-hemoglobin (> 50% each) compared to normal breast tissues. Functional perturbations by the tumor were significantly larger than functional variations in normal tissues. A tissue optical index (TOI) derived from intrinsic physiological properties yielded an average two-fold contrast difference between malignant tumors and intrinsic tissue properties. Our results demonstrate that intrinsic optical signals can be influenced by functional perturbations characteristic of malignant transformation; cellular metabolism, extracellular matrix composition, and angiogenesis. Our findings further underscore the importance of broadband measurements and patient age stratification in breast cancer DOI. (c) 2006 Society of Photo-Optical Instrumentation Engineers.