Isolation and characterization of revertant cell lines. IV. Direct selection of serum‐revertant sublines of SV40‐transformed 3T3 mouse cells

Isolation and characterization of revertant cell lines. IV. Direct selection of serum‐revertant sublines of SV40‐transformed 3T3 mouse cells
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IV. SV40 转化的 3T3 小鼠细胞血清回复亚系的分离和表征。

DOI:
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发表时间:
1973
影响因子:
5.6
通讯作者:
R. Pollack
R. Pollack
中科院分区:
生物学2区
文献类型:
--
作者:
A. Vogel;R. Pollack

文献摘要

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SV101是小鼠成纤维细胞系3T3的SV40转化亚系,在1%和10%的小牛血清中生长,在10%的小牛血清中生长,在血清和密度上均有转化。在低细胞密度的1%小牛血清或10%去甲胎血清中的负选择允许直接恢复血清逆转的SV101亚系。这些亚系在1%小牛血清中不能生长。
SV101, the SV40‐transformed subline of the mouse fibroblast line 3T3, is both serum‐ and density transformed, since it grows in both 1% and 10% calf serum, and grows beyond confluence in 10% calf serum. Negative selection at low cell density in 1% calf serum or in 10% agamma‐depleted serum permits direct recovery of serum‐revertant sublines of SV101. These sublines are unable to grow in 1% calf serum.