Technical improvement of human pancreatic islet isolation.
Technical improvement of human pancreatic islet isolation.
复制标题
人胰岛分离技术的改进。
DOI：
10.1097/00007890-200407271-00932
复制
发表时间：
2004
影响因子：
0.9
通讯作者：
O. Korsgren
中科院分区：
文献类型：
--
作者：
M. Goto;T. Eich;M. Ståhle;A. Malmborg;M. Engkvist;O. Korsgren
介绍
成功分离胰岛的关键因素是在开始消化胰腺之前将最佳量的酶放置到胰管中。为了改进这一过程，我们引入了新的技术来识别和修复导致胶原酶溶液泄漏的组织损伤。
材料和方法
112个标准化的连续胰岛分离是为了研究染料和胶对胰岛产量、使用灌注测定的胰岛功能以及临床移植的可能性的影响。其中一组（n = 26）胰腺与十二指肠在隔离单元中通过结扎副胰管小心地分离（WPD组），而另一组（n = 86）胰腺在手术室中与十二指肠分离。在86个分离物中的28个中，使用整个腺体（WP组），而在剩余的58个分离物中仅施用身体和尾部区域（PP组）。
结果
染料和胶水都有效地防止了胶原酶从腺体中泄漏。使用这些工具，胰岛产量和成功率均较高，对胰岛功能或胶原酶活性无不良影响。WPD组的分离成功率和胰岛产量显著较高（分别为P = 0.02和0.001）。
结论
染料和胶水可能是改善人类胰岛分离程序的有用工具。此外，使用整个胰腺进一步改善了结果。
INTRODUCTION
A key factor for successful islet isolation is to place the optimal amount of enzyme into the pancreatic ducts prior to starting digestion of pancreatic glands. To improve this procedure, we introduced novel techniques to identify and repair tissue damage resulting in leakage of collagenase solution.
MATERIALS AND METHODS
One hundred twelve standardized consecutive islet isolations were for the effects of dye and glue on islet yield, islet function using a perifusion assay, and the possibility of clinical transplantation. One group of pancreata (n = 26) obtained en bloc together with duodenum were carefully detached with ligation of accessory ducts in an isolation unit (WPD group), whereas the pancreata were dissected from the duodenum in the operating room in the other 86 isolations. In 28 of 86 isolations, whole glands were used (WP group), while only the body and tail area were applied in the remaining 58 isolations (PP group).
RESULTS
Both dye and glue effectively prevented leakage of collagenase from the gland. Both islet yield and success rate were higher with these tools without adverse effects on islet function or collagenase activity. The success rate of isolations and islet yield were significantly higher in the WPD group (P = .02 and .001, respectively).
CONCLUSIONS
Dye and glue may be useful tools to improve human islet isolation procedures. In addition, the use of the whole pancreas further improves the outcome.