A convenient purification method for silver nanoclusters and its applications in fluorescent pH sensors for bacterial monitoring.
A convenient purification method for silver nanoclusters and its applications in fluorescent pH sensors for bacterial monitoring.
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DOI：
10.1016/j.bios.2016.06.038
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发表时间：
2016-12
影响因子：
12.6
通讯作者：
Huayu Xiong;Huiling Zheng;Wei Wang;Jichao Liang;Wei Wen;Xiuhua Zhang;Shengfu Wang
中科院分区：
文献类型：
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作者：
Huayu Xiong;Huiling Zheng;Wei Wang;Jichao Liang;Wei Wen;Xiuhua Zhang;Shengfu Wang
在此，我们报告了一种在酸性条件下通过ph诱导沉淀纯化水溶性二氢硫辛酸（DHLA）覆盖的银纳米簇（Ag NCs）的简便方法。用DHLA和nabh4作为稳定配体和蚀刻试剂，将沉淀重新分散到碱性溶液中，可以完全恢复Ag nc的荧光。制备了DHLA-Ag ncs掺杂琼脂糖水凝胶，用于监测pH值，范围从8.0到4.0。当pH降低时，水凝胶在紫外灯下的荧光减弱，pH 5后荧光完全消失。DHLA-Ag - ncs掺杂琼脂糖水凝胶生物传感器具有低细胞毒性和长稳定性。因此，基于Ag ncs -琼脂糖水凝胶的“OFF-ON”信号开关，采用了一种用于细菌监测的荧光pH传感器。
Herein, we report a convenient approach to purify water-soluble dihydrolipoic acid (DHLA)-capped Ag nanoclusters (Ag NCs) by pH-induced precipitation under acidic conditions. The fluorescence of Ag NCs could be completely recovered by re-dispersing the precipitate into a basic solution using DHLA and NaBH4as stabilizing ligands and etching reagent. DHLA-Ag NCs-doped agarose hydrogels have been prepared to monitor pH with a wide range from 8.0 to 4.0. When pH decreased, the fluorescence of the hydrogels under a UV lamp decreased and completely disappeared after pH 5. The DHLA-Ag NCs-doped agarose hydrogels biosensor showed low cytotoxicity and long stability. Accordingly, a fluorescent pH sensor for bacterial monitoring has been employed based on the “OFF–ON” signal switch of the Ag NCs-agarose hydrogel.